Effect of Arsenic on Nitrosative Stress in Human Breast Cancer Cells and its Modulation by Flavonoids

Authors
Soria, Elio Andres; Bongiovanni, Guillermina Azucena; Díaz, Luján Cintia; Eynard, Aldo Renato
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Arsenic (As) is used in the treatment of leukaemia and breast cancer due to its strong oxidative cytotoxic action. However, it is also cytotoxic to normal cells. One proposed anticancer mechanism induced by As might be nitrosative stress (NS). Hence, it is believed that use of antioxidants such as flavonoids in combination with As might reduce its toxic action on normal cells without interfering with its antitumor action. In the present study, we evaluated the antineoplastic potential of As on breast human cancer lines MCF-7 and ZR- 75-1 treated with redox-modulating flavonoids, such as quercetin (Q) and silymarin (S). It was noted that, even though both cell lines differed in their oxidative responsiveness, their viability was decreased by NS induction through -glutamyltranspeptidase inhibition. Arsenic triggered NS in both MCF-7 and ZR-75-1 cultures, with the formers being more sensitive without recovering their pre-treatment capacity. ZR-75-1 cells maintained their antioxidant status, whereas MCF-7 ones treated with S, As and As+Q did not. Silymarin did not interfere with the described As bioactivity. In summary, NS appears as an important anticancer mechanism exerted by As depending on the redox cellular response that could be differentially modified by dietary antioxidants. Hence, it is worthwhile to consider the use of dietary antioxidants as adjuvant in cancer chemotherapy, especially when using As.
Fil: Soria, Elio Andres. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Bongiovanni, Guillermina Azucena. Universidad Nacional del Comahue; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Díaz, Luján Cintia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Eynard, Aldo Renato. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Cordoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Subject
ARSENIC
BREAST CANCER
FLAVONOID
NITROSATIVE STRESS
QUERCETIN
SILYMARIN
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/9856