Toxicogenomics: new strategies for ecotoxicology studies in autochthonous species II : The 'omic' era in non-model species : Transcriptome analysis for biomarker screening

Authors
Ceschin, Danilo Guillermo
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
The emerging field of ecotoxicogenomics aims to combine large-scale approaches to study the responses of organisms to a toxicant. A holistic vision of gene and protein expression in response to toxic exposure contributes to the identification of cellular components, signalling pathways and novel mechanisms of action/response. Native species are preferential for evaluating the impact of contaminants generated by anthropic action. However, biomonitoring using autochthonous species (non-model organisms) is difficult owing to a deficiency of molecular biology data and analytical tools. Our experience in the study of biomarkers in the South American toad Rhinella arenarum revealed many difficulties of finding antibodies or designing probes for it. We performed a transcriptomic study in R. arenarum exposed to two organophosphorus pesticides. We determined that there are specific patterns of gene expression for each organophosphate tested. Thus, a transcriptome approach for biomarker screening seems to be helpful in defining specific gene expression behaviour for a given toxicant.
Fil: Ceschin, Danilo Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
BIOMARKER
ECOTOXICOGENOMICS
NEXT-GENERATION SEQUENCING
NGS
NON-MODEL ORGANISM
RHINELLA ARENARUM
RNA-SEQ
SYSTEM BIOLOGY
TOXICOGENOMICS
TRANSCRIPTOME
Ciencias Medioambientales
Ciencias de la Tierra y relacionadas con el Medio Ambiente
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/58218