Acinetobacter baumannii extensively drug resistant lineages in Buenos Aires hospitals differ from the international clones I–III

Authors
Stietz, Maria Silvina; Ramirez, Maria Soledad; Vilacoba, Elisabet; Merkier, Andrea Karina; Limansky, Adriana Sara; Centrón, Daniela; Catalano, Mariana
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
As a way to contribute to the assessment of Acinetobacter baumannii clinical population structure, multilocus sequence typing (MLST) was performed in a collection of 93 isolates from Buenos Aires (1983? 2012) and Rosario (2006?2009) hospitals. Sequence types (STs) were achieved by Bartual (B) and Institut Pasteur (P) schemes. PFGE typing, antimicrobial susceptibility assays, and the amplification of the OXA carbapenemase genes most prevalent in our region, were also performed. e-Burst clustered the 25 STsB (15 novels) into 5 clonal complexes (CC) and 5 singletons, and grouped the 18 STsP (12 novels) into 3 CC and 4 singletons. Bartual scheme divided the CC79P into two groups. CC113B/CC79P prevailed in Buenos Aires at least in 1992?2009, being responsible for epidemic and for endemic infections and acquiring the XDR (extensively drug-resistant) pattern throughout the years. While, CC119B/CC79P was apparently present before the CC113B/CC79Pdomain. CC103B/CC15P was the second most prevalent CC. Interestingly, CC110B/ST25P apparently increased over the last years. Conversely, CC109B/CC1P (international clone I) predominated in Rosario, although the presence of CC113B/CC79P, CC103B/CC15P and CC110B/ST25P was observed. Nineteen novel STs clustered in CC79P, CC15P, CC113B, CC109B and CC103B, suggesting their clonal expansion during persistence. PFGE typing proved transmission of strains intra- and inter-hospitals in each city. Except for one, all the recent isolates (2007?2012) harboured the blaOXA-23-like. All isolates were susceptible to colistin. Tigecycline MIC90 was 1 mg/L and the rifampicin MIC > 512 mg/l was found among isolates in three hospitals. In conclusion, the international clone II (CC92B/CC2P) was not found among our isolates. CC113B/CC79P, CC103B/CC15P, and ST25P, suggested also as major components in the A. baumannii population together with the international clone I, were present in Buenos Aires and Rosario with different prevalence rate. Their recent isolates showed high distribution of the blaOXA-23-like as well as the XDR pattern.
Fil: Stietz, Maria Silvina. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Microbiología. Cátedra de Microbiología, Parasitología e Inmunología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Fil: Ramirez, Maria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Fil: Vilacoba, Elisabet. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Fil: Merkier, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Fil: Limansky, Adriana Sara. Universidad Nacional de Rosario; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina;
Fil: Centrón, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Fil: Catalano, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina;
Subject
Acinetobacter baumannii
CLONES INTERNACIONALES
Enfermedades Infecciosas
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Genética y Herencia
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1899