Micropropagation of Handroanthus heptaphyllus (Vell.) Mattos from seedling explants

Authors
Duarte, Evelyn Raquel; Sansberro, Pedro Alfonso; Luna, Claudia Verónica
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Handroanthus heptaphyllus (Vell.) Mattos (Bignoniaceae), a tropical forest tree, is a source of wood suitable for the manufacture of fine furniture and chemical compounds with medicinal and insecticidalproperties. Poor seed viability of this species limits the conventional propagation practice. In vitro germination of seeds was achieved in semi-solid Murashige and Skoog?s (MS) medium. A disinfectionprotocol was optimized for germination and growth of seedlings in vitro. Proliferation of shoots from cotyledonary and stem nodal segments was achieved in semi-solid MS medium supplemented with 22.2 μM 6-benzyladenine. Temporary immersion culture promoted elongation and rooting of shoots andreduced the shoot tip necrosis compared with culture on semisolid medium (6.7±2.7 and 41.7±7.0%,respectively; P = 0.001). Plantlets derived from temporary immersion showed better performance during the acclimatization phase. This is the first report on micropropagation of H. heptaphyllus.
Fil: Duarte, Evelyn Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Fil: Luna, Claudia Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
Subject
Handroanthus heptaphyllus
Axillary shoots
Adventitious shoots
Micropropagation
Seed
Uncoated seeds
Cotyledon
Stem nodes
Black lapacho
Biotecnología Agrícola y Biotecnología Alimentaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Open access
License
https://creativecommons.org/licenses/by/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/20648