An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel

Authors
Peirú, Salvador; Aguirre, Andres; Eberhardt, Maria Florencia; Braia, Mauricio Javier; Cabrera, Rodolfo Ariel; Menzella, Hugo Gabriel
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Background: Biodiesels produced from transesterification of vegetable oils have a major quality problem due to thepresence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitateshave been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods toremove these compounds are not available. Here we describe a novel method for the efficient removal of SGs frombiodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis.Results: A steryl glucosidase (SGase) enzyme from T. litoralis was produced and purified from Escherichia coli culturesexpressing a synthetic gene, and used to treat soybean-derived biodiesel. Several optimization steps allowed for theselection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs fromcrude biodiesel. The resulting biodiesel displayed filterability properties similar to distilled biodiesel according to thetotal contamination (TC), the cold soak filtration test (CSFT), filter blocking tendency (FBT), and cold soak filter blockingtendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability toindustrial settings.Conclusions: The results presented in this work provide a novel path for the removal of steryl glucosides from biodieselusing a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption ofthis renewable fuel.
Fil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Aguirre, Andres. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Eberhardt, Maria Florencia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Braia, Mauricio Javier. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera, Rodolfo Ariel. Unitec Bio; Argentina
Fil: Menzella, Hugo Gabriel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Keclon; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
SYNTHETIC BIOLOGY
GREEN CHEMISTRY
BIOFUELS
Biotecnología Industrial
Biotecnología Industrial
INGENIERÍAS Y TECNOLOGÍAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/42879