1H, 13C and 15N resonance assignments of human parvulin 17

Authors
Lin, Yi Jan; Schmidt, Andreas; Burgardt, Noelia Ines; Thiele, Alexandra; Weiwad, Matthias; Lücke, Christian
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
A 25-residue elongation at the N-terminus endows parvulin 17 (Par17) with altered functional properties compared to parvulin 14 (Par14), such as an enhanced influence on microtubule assembly. Therefore the three-dimensional structure of this N-terminal elongation is of particular interest. Here, we report the nearly complete 1H, 13C and 15N chemical shift assignments of Par17. Subsequent chemical shift index analysis indicated that Par17 features a parvulin-type PPIase domain at the C-terminus, analogous to Par14, and an unstructured N-terminus encompassing the first 60 residues. Hence the N-terminus of Par17 apparently adopts a functionally-relevant structure only in presence of the respective interaction partner(s).
Fil: Lin, Yi Jan. Kaohsiung Medical University; China
Fil: Schmidt, Andreas. Max Planck Research Unit for Enzymology of Protein Folding; Alemania
Fil: Burgardt, Noelia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina. Max Planck Research Unit for Enzymology of Protein Folding; Alemania
Fil: Thiele, Alexandra. Max Planck Research Unit for Enzymology of Protein Folding; Alemania
Fil: Weiwad, Matthias. Max Planck Research Unit for Enzymology of Protein Folding; Alemania
Fil: Lücke, Christian. Max Planck Research Unit for Enzymology of Protein Folding; Alemania
Subject
PPIase
Par14
Par17
DNA binding
Microtubule assembly
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/18092