Pseudovitamin B12 is the corrinoid produced by Lactobacillus reuteri CRL1098 under anaerobic conditions

Authors
Santos, Felipe; Vera, José Luis; Lamosa, Pedro; Font, Graciela Maria; de Vos, Willem M.; Santos, Helena; Sesma, Fernando Juan Manuel; Hugenholtz, Jeroen
Publication Year
2007
Language
English
Format
article
Status
Published version
Description
We have reported previously on the ability of Lactobacillus reuteri to produce a compound with vitamin B12 activity. Here we report on the chemical characterisation of this corrinoid-like molecule. High performance liquid chromatography coupled to an ultraviolet diode array detector, mass spectrometry and nuclear magnetic resonance spectroscopy has enabled us to identify the compound as Coα-[α-(7-adenyl)]-Coβ-cyanocobamide or pseudovitamin B12. This molecule differs from cobalamin in the α-ligand, where it has adenine instead of 5,6-dimethylbenzimidazole bound in a α-glycosidic linkage to C-1 of ribose. L. reuteri is the first lactic acid bacterium in which the production of a cobalamin-like molecule has been identified and the first microorganism reported to produce exclusively pseudo-B12. © 2007 Federation of European Biochemical Societies.
Fil: Santos, Felipe. NIZO Food Research; Países Bajos
Fil: Vera, José Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Lamosa, Pedro. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Font, Graciela Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: de Vos, Willem M.. Wageningen University and Research Centre.Laboratory of Microbiology ; Países Bajos
Fil: Santos, Helena. Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica; Portugal
Fil: Sesma, Fernando Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Hugenholtz, Jeroen. NIZO Food Research; Países Bajos
Subject
B12 ANALOGUES
COBAMIDE
CORRINOIDS
LACTIC ACID BACTERIA
LACTOBACILLUS REUTERI
PSEUDOVITAMIN B12
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/58580