Acetohydroxyacid synthase activity and transcripts profiling reveal tissue-specific regulation of ahas genes in sunflower

Authors
Ochogavía, Ana Claudia; Breccia, Gabriela; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Picardi, Liliana Amelia; Nestares, Graciela María
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Acetohydroxyacid synthase (AHAS) is the target site of several herbicides and catalyses the first step in the biosynthesis of branched chain amino acid. Three genes coding for AHAS catalytic subunit (ahas1, ahas2 and ahas3) have been reported for sunflower. The aim of this work was to study the expression pattern of ahas genes family and AHAS activity in sunflower (Helianthus annuus L.). Different organs (leaves, hypocotyls, roots, flowers and embryos) were evaluated at several developmental stages. The transcriptional profile was studied through RT-qPCR. The highest expression for ahas1 was shown in leaves, where all the induced and natural gene mutations conferring herbicide resistance were found. The maximal expression of ahas2 and ahas3 occurred in immature flowers and embryos. The highest AHAS activity was found in leaves and immature embryos. Correlation analysis among ahas gene expression and AHAS activity was discussed. Our results show that differences in ahas genes expression are tissue-specific and temporally regulated. Moreover, the conservation of multiple AHAS isoforms in sunflower seems to result from different expression requirements controlled by tissue-specific regulatory mechanisms at different developmental stages
Fil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vega, Tatiana Alejandra. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Picardi, Liliana Amelia. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina. Universidad Nacional de Rosario. Consejo de Investigaciones; Argentina
Fil: Nestares, Graciela María. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Departamento de Biologia. Cat.de Genetica; Argentina
Subject
ACETOHYDROXYACID SYNTHASE
AHAS ACTIVITY
AHAS GENES
GENE EXPRESSION
HELIANTHUS ANNUUS L.
SUNFLOWER
Agricultura
Agricultura, Silvicultura y Pesca
CIENCIAS AGRÍCOLAS
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/29912