Development and testing of an optimized method for DNA-based identification of jaguar (Panthera onca) and puma (Puma concolor) faecal samples for use in ecological and genetic stud...

Authors
Haag, Taiana; Santos, Analisie S.; de Angelo, Carlos Daniel; Srbek-Araujo, Ana Carolina; Sana, Dênis A.; Morato, Ronaldo G.; Salzano, Francisco M.; Eizirik, Eduardo
Publication Year
2009
Language
English
Format
article
Status
Published version
Description
The elusive nature and endangered status of most carnivore species imply that efficient approaches for their non-invasive sampling are required to allow for genetic and ecological studies. Faecal samples are a major potential source of information, and reliable approaches are needed to foster their application in this field, particularly in areas where few studies have been conducted. A major obstacle to the reliable use of faecal samples is their uncertain species-level identification in the field, an issue that can be addressed with DNA-based assays. In this study we describe a sequence-based approach that efficiently distinguishes jaguar versus puma scats, and that presents several desirable properties: (1) considerably high amplification and sequencing rates; (2) multiple diagnostic sites reliably differentiating the two focal species; (3) high information content that allows for future application in other carnivores; (4) no evidence of amplification of prey DNA; and (5) no evidence of amplification of a nuclear mitochondrial DNA insertion known to occur in the jaguar. We demonstrate the reliability and usefulness of this approach by evaluating 55 field-collected samples from four locations in the highly fragmented Atlantic Forest biome of Brazil and Argentina, and document the presence of one or both of these endangered felids in each of these areas. © 2009 Springer Science+Business Media B.V.
Fil: Haag, Taiana. Universidade Federal do Rio Grande do Sul; Brasil. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: Santos, Analisie S.. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Fil: de Angelo, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Centro de Investigaciones del Bosque Atlántico; Argentina
Fil: Srbek-Araujo, Ana Carolina. Instituto Ambiental Vale, Vitoria; Brasil
Fil: Sana, Dênis A.. Instituto Pró-Carnívoros, Atibaia; Brasil
Fil: Morato, Ronaldo G.. Instituto Pró-Carnívoros, Atibaia; Brasil. CENAP/ICMBio, Atibaia; Brasil
Fil: Salzano, Francisco M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Eizirik, Eduardo. Laboratory of Genomic Diversity, NCI, Frederick; Estados Unidos. Instituto Pró-Carnívoros, Atibaia; Brasil. Pontificia Universidade Católica do Rio Grande do Sul; Brasil
Subject
FAECAL DNA
MITOCHONDRIAL DNA
PANTHERA ONCA
PUMA CONCOLOR
SPECIES IDENTIFICATION
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/60507