A protocol for the in vitro propagation and polyploidization of an interspecific hybrid of Glandularia (G. peruviana×G. scrobiculata)

Authors
González Roca, Lucía; Iannicelli, Jesica; Coviella, Andrea; Bugallo, Verónica; Bologna, Paula; Pitta Alvarez, Sandra Irene; Escandón, Alejandro Salvio
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
The genus Glandularia (Verbenaceae) has more than 50 species and it holds great ornamental potential due to its colorful flowers, long flowering period and low water requirements. Since the floriculture market avidly seeks novelties, increasing the diversity within the genus would increase its commercial value. Some of the traits that should be improved in these species are their architecture and the size and colors of the flowers and leaves. In this sense, obtaining polyploid individuals is an interesting strategy to achieve this objective. In this paper a hybrid of Glandularia peruviana×Glandularia scrobiculata was cultured under in vitro conditions. Different plant growth regulators and their combinations were tested to obtain appropriate multiplication rates. The best results were obtained with the combination of 6.6μM thidiazuron/0.03μM α-naphthalenacetic acid, with a multiplication rate of 19 shoots per explant. The plantlets were first rooted and then acclimatized by transferal to an 8.0cm diameter pot containing Growing mix®, and maintenance inside a humidity chamber. For poliploydization, the explants were exposed to colchicine in concentrations of 0.001% and 0.01% for 24 and 48h. The 40 recovered plants were characterized according to their DNA content. There were 21 diploids, 14 solid tetraploids, 1 solid octoploid, 3 chimera tetraploids and 1 chimera octoploid. Phenotypically, the size of the flowers, inflorescences, pollen grains and stomata were significantly larger in polyploid individuals. Surprisingly, stem diameter, leaf size and color intensity of the leaves and flowers were not significantly different between diploid and tetraploid individuals. Due to the size of the inflorescences, the tetraploid individuals are a promising starting material for a Glandularia breeding program.
Fil: González Roca, Lucía. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Iannicelli, Jesica. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coviella, Andrea. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Bugallo, Verónica. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Bologna, Paula. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Floricultura; Argentina
Fil: Pitta Alvarez, Sandra Irene. Universidad Argentina de la Empresa; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Escandón, Alejandro Salvio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Subject
BREEDING
ORNAMENTAL PLANTS
PLANT BIOTECHNOLOGY
TISSUE CULTURE
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/37698