Superoxide dismutase content in sperm correlates with motility recovery after thawing of cryopreserved human spermatozoa

Authors
Buffone, Mariano Gabriel; Calamera, Juan C.; Brugo Olmedo, Santiago; de Vincentiis, Sabrina; Calamera, María M.; Storey, Bayard T; Doncel, Gustavo F.; Alvarez, Juan G.
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
Objective To investigate the correlation between sperm superoxide dismutase (SOD) content and motility recovery after thawing of cryopreserved human sperm, based on the rationale that this antioxidant enzyme provides protection against reactive oxygen species-induced damage during cryopreservation. Design Prospective study. Setting Private infertility institute and university-based research laboratory. Patient(s) Forty-two consenting normozoospermic patients consulting for infertility. Intervention(s) The SOD content was measured in sperm from unfractionated samples and in sperm recovered from the pellet fraction obtained after discontinuous density gradient centrifugation. Main Outcome Measure(s) Sperm motility was evaluated post-thaw in the two sets of samples and motility recovery was plotted against the sperm SOD content to determine their correlation. Result(s) There was a significant positive correlation between motility recovery after thawing and SOD content in sperm from the 90% gradient pellet containing highly purified mature sperm. There was also a significant negative correlation between motility after thawing and SOD content in the unfractionated sample. Conclusion(s) The positive correlation between post-thaw motility recovery and SOD content in mature spermatozoa provides a good predictor of post-thaw motility recovery after cryopreservation.
Fil: Buffone, Mariano Gabriel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Calamera, Juan C.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Brugo Olmedo, Santiago. Centro Médico Seremas; Argentina
Fil: de Vincentiis, Sabrina. Centro Médico Seremas; Argentina
Fil: Calamera, María M.. Laboratorio de Estudios en Reproducción; Argentina
Fil: Storey, Bayard T. University of Pennsylvania; Estados Unidos
Fil: Doncel, Gustavo F.. Eastern Virginia Medical School. Jones Institute For Reproductive Medicine; Estados Unidos
Fil: Alvarez, Juan G.. Centro ANDROGEN; España. Harvard Medical School; Estados Unidos
Subject
CRYOPRESERVATION
SPERM
SOD
ROS
SPERM MOTILITY
Biología Reproductiva
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/8773