Antimicrobial properties of silver nanoparticles against biofilm formation by Pseudomonas aeruginosa on archaeological textiles

Authors
Pietrzak, Katarzyna; Otlewska, Anna; Puchalski, Michal; Gutarowska, Beata; Guiamet, Patricia Sandra
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
The aims of this work were to: (i) microscopically analyse the pre- and post-Columbian archaeological textiles using Scanning Electron Microscopy with Energy Dispersive X-Ray Analysis (SEM-EDX); (ii) microbiologically analyse the archaeological textiles (from the Southern Andean Area, La Plata Museum); (iii) determine the ability of Pseudomonas sp. isolates from archaeological textiles to biofilm formation by SEM; (iv) assess the anti-biofilm properties of AgNPs protecting cotton against Pseudomonas sp. Results showed the presence of bacteria with proteolytic and lipolytic activities on archaeological textiles, including Clostridium sp. and Pseudomonas aeruginosa. Two nucleotide sequences of 16S ribosomal RNA gene of P. aeruginosa strains were deposited in GeneBank NCBI database with accession numbers: KP842564 (strain 1) and KP842565 (strain 2). Those strains exhibited different morphological and growth characteristics: strain 1 with ability to form biofilms on archaeological textiles was rod-shaped, produced bluish-green pigment, and smaller than strain 2; and strain 2 was pleomorphic and produced brown pigment. The use of silver nanoparticles (90 ppm, φ 10–80 nm) allowed to protecting textiles against P. aeruginosa growth by 63%–97%, depending on the strain and exposition time.
Fil: Pietrzak, Katarzyna. Lodz University of Technology; Polonia
Fil: Otlewska, Anna. Lodz University of Technology; Polonia
Fil: Puchalski, Michal. Lodz University of Technology; Polonia
Fil: Gutarowska, Beata. Lodz University of Technology; Polonia
Fil: Guiamet, Patricia Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Subject
ARCHAEOLOGICAL TEXTILES
PSEUDOMONAS sp.
PROTECTION
SILVER NANOPARTICLES
BIOFILM
SEM-EDX
Bioremediación, Diagnóstico Biotecnológico en Gestión Medioambiental
Biotecnología del Medio Ambiente
INGENIERÍAS Y TECNOLOGÍAS
Historia
Historia y Arqueología
HUMANIDADES
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/64092