Second harmonic generation microscopy as a powerful diagnostic imaging modality for human ovarian cancer

Authors
Adur, Javier Fernando; Pelagati, Vitor B.; de Thomaz, Andre A.; Baratti, Mariana O.; Andrade, Liliana A. L. A.; Carvalho, Hernandes F.; Bottcher Luiz, Fátima; Lenz Cesar, Carlos
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
In this study we showed that second-harmonic generation (SHG) microscopy combined with precise methods for images evaluation can be used to detect structural changes in the human ovarian stroma. Using a set of scoring methods (alignment of collagen fibers, anisotropy, and correlation), we found significant differences in the distribution and organization of collagen fibers in the stroma component of serous, mucinous, endometrioid and mixed ovarian tumors as compared with normal ovary tissue. This methodology was capable to differentiate between cancerous and healthy tissue, with clear cut distinction between normal, benign, borderline, and malignant tumors of serous type. Our results indicated that the combination of different image-analysis approaches presented here represent a powerful tool to investigate collagen organization and extracellular matrix remodeling in ovarian tumors.
Fil: Adur, Javier Fernando. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina. Universidade Estadual de Campinas; Brasil. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pelagati, Vitor B.. Universidade Estadual de Campinas; Brasil
Fil: de Thomaz, Andre A.. Universidade Estadual de Campinas; Brasil
Fil: Baratti, Mariana O.. Universidade Estadual de Campinas; Brasil
Fil: Andrade, Liliana A. L. A.. Universidade Estadual de Campinas; Brasil
Fil: Carvalho, Hernandes F.. Universidade Estadual de Campinas; Brasil
Fil: Bottcher Luiz, Fátima. Universidade Estadual de Campinas; Brasil
Fil: Lenz Cesar, Carlos. Universidade Estadual de Campinas; Brasil
Subject
SECOND HARMONIC GENERATION MICROSCOPY
OVARIAN EPITHELIAL TUMORS
COLLAGEN QUANTIFICATION
EXTRACELLULAR MATRIX
Astronomía
Ciencias Físicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/32616