A scientific note on the first report of honeybee venom inhibiting Paenibacillus larvae growth

Authors
Fernández, Natalia Jorgelina; Porrini, Martín Pablo; Podaza, Enrique Arturo; Damiani, Natalia; Gende, Liesel Brenda; Eguaras, Martin Javier
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
In the eusocial honeybee, Apis mellifera, worker bees use a stinging apparatus for defense. The sting is upplied with venom by glands localized in the abdomen. Honeybee venom (BV) is composed of at least 18 bioactive molecules, ranging from biogenic amines to proteins whose structure and function have been largely determined. These include peptides such as melittin, apamin, adolapin, and mast cell degranulating peptide; biologically active amines; enzymes as phospholipase A2 (PLA2); and a few nonpeptide components (Peiren et al. 2005;Matysiak et al. 2011).Melittin and PLA2 are the most abundant proteins, representing 50 and 12 % of BV dry weight, respectively. This research also constitutes the first record of oral administration of BV to an invertebrate biological model. No lethal or behavioral effects on bees after a week of ingesting BV were revealed. High doses of BV proved much less toxic for bees than some botanical extracts (Damiani et al. 2014). Further studies should be conducted to determine the toxicological risks and the optimum dosage of BV or the use of single antimicrobial peptides (i.e., Melittin) for field assays. BVextraction has become a standardized practice, characterized for being safe, simple, and causing no harm to bees. It would be interesting to investigate its effect on other apicultural diseases.
Fil: Fernández, Natalia Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Porrini, Martín Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Podaza, Enrique Arturo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Damiani, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Gende, Liesel Brenda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Eguaras, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales; Argentina
Subject
honeybee venom
Paenibacillus larvae
antimicrobial activity
Apis mellifera
Otras Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/34581