Distributed structures underlie gating differences between the K in Channel KAT1 and the K out Channel SKOR

Authors
Riedelsberger, Janin; Sharma, Tripti; Gonzalez, Wendy; Gajdanowicz, Pawel; Morales Navarro, Samuel Elías; Garcia-Mata, Carlos; Mueller Roeber, Bernd; González Nilo, Fernando Danilo; Blatt, Michael R.; Dreyer, Ingo
Publication Year
2010
Language
English
Format
article
Status
Published version
Description
The family of voltage-gated (Shaker-like) potassium channels in plants includes both inward-rectifying (Kin) channels that allow plant cells to accumulate K+ and outward-rectifying (Kout) channels that mediate K+ efflux. Despite their close structural similarities, Kin and Kout channels differ in their gating sensitivity towards voltage and the extracellular K+ concentration. We have carried out a systematic program of domain swapping between the Kout channel SKOR and the Kin channel KAT1 to examine the impacts on gating of the pore regions, the S4, S5, and the S6 helices. We found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. Our findings were supported by molecular dynamics of KAT1 and SKOR homology models. In silico analysis revealed that during channel opening and closing, displacement of certain residues, especially in the S5 and S6 segments, is more pronounced in KAT1 than in SKOR. From our analysis of the S4–S6 region, we conclude that gating (and K+-sensing in SKOR) depend on a number of structural elements that are dispersed over this ∼145-residue sequence and that these place additional constraints on configurational rearrangement of the channels during gating.
Fil: Riedelsberger, Janin . Universität Potsdam; Alemania. Max-Planck Institute of Molecular Plant Physiology; Alemania
Fil: Sharma, Tripti . Universität Potsdam; Alemania. Max-Planck Institute of Molecular Plant Physiology; Alemania
Fil: Gonzalez, Wendy . Universidad de Talca; Chile
Fil: Gajdanowicz, Pawel . Universität Potsdam; Alemania
Fil: Morales Navarro, Samuel Elías . Universidad de Talca; Chile
Fil: Garcia-Mata, Carlos. University Of Glasgow; Reino Unido. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mueller Roeber, Bernd. Max-Planck Institute of Molecular Plant Physiology; Alemania. Universität Potsdam; Alemania
Fil: González Nilo, Fernando Danilo . Universidad de Talca; Chile
Fil: Blatt, Michael R. . University Of Glasgow; Reino Unido
Fil: Dreyer, Ingo . Universität Potsdam; Alemania
Subject
Arabidopsis
K+ channel
outward rectifier
inward rectifier
channel protein structure
channel protein–cation interaction
gating
K+-dependent
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/13211