Exopolysaccharides from Lactobacillus delbrueckii OLL1073R-1 modulate innate antiviral immune response in porcine intestinal epithelial cells

Authors
Kanmani, Paulraj; Albarracín, Leonardo Miguel; Kobayashi, Hisakazu; Iida, Hikaru; Komatsu, Ryoya; Kober, Humayun A. K. M.; Ikeda-Ohtsubo, Wakako; Suda, Yoshihito; Aso, Hisashi; Makino, Seiya; Kano, Hiroshi; Saito, Tadao; Villena, Julio Cesar; Kitazawa, Haruki
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
Previous studies demonstrated that the extracellular polysaccharides (EPSs) produced by Lactobacillus delbrueckii OLL1073R-1 (LDR-1) improve antiviral immunity, especially in the systemic and respiratory compartments. However, it was not studied before whether those EPSs are able to beneficially modulate intestinal antiviral immunity. In addition, LDR-1-host interaction has been evaluated mainly with immune cells while its interaction with intestinal epithelial cells (IECs) was not addressed before. In this work, we investigated the capacity of EPSs from LDR-1 to modulate the response of porcine IECs (PIE cells) to the stimulation with the Toll-like receptor (TLR)-3 agonist poly(I:C) and the role of TLR2, TLR4, and TLR negative regulators in the immunoregulatory effect. We showed that innate immune response triggered by TLR3 activation in porcine IECs was differentially modulated by EPS from LDR-1. EPSs treatment induced an increment in the expression of interferon (IFN)-α and IFN-β in PIE cells after the stimulation with poly(I:C) as well as the expression of the antiviral factors MxA and RNase L. Those effects were related to the reduced expression of A20 in EPS-treated PIE cells. EPS from LDR-1 was also able to reduce the expression of IL-6 and proinflammatory chemokines. Although further in vivo studies are needed, our results suggest that these EPSs or a yogurt fermented with LDR-1 have potential to improve intestinal innate antiviral response and protect against intestinal viruses.
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Iida, Hikaru. Tohoku University; Japón
Fil: Komatsu, Ryoya. Tohoku University; Japón
Fil: Kober, Humayun A. K. M.. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University. Department of Dairy & Poultry Science; Bangladesh
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environment; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Makino, Seiya. Meiji Co., Ltd. Food Science Research Laboratory; Japón
Fil: Kano, Hiroshi. Meiji Co., Ltd. Food Science Research Laboratory; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Subject
ANTI-INFLAMMATORY ACTIVITY
ANTIVIRAL ACTIVITY
EXOPOLYSACCHARIDES
IMMUNE MODULATION
PORCINE INTESTINAL EPITHELIAL CELLS
PROBIOTICS
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/58988