Whole-Genome Comparative Analysis of Two Carbapenem-Resistant ST-258 Klebsiella pneumoniae Strains Isolated during a North-Eastern Ohio Outbreak: Differences within the High Hetero...

Authors
Ramirez, Maria Soledad; Xie, Gang; Traglia, German Matias; Johnson, Shannon L.; Davenport, Karen W.; Van Duin, David; Ramazani, Azam; Perez, Federico; Jacobs, Michael R.; Sherratt, David J.; Bonomo, Robert A.; Chain, Patrick S. G.; Tolmasky, Marcelo E.
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Klebsiella pneumoniae has become one of the most dangerous causative agents of hospital infections due to the acquisition of resistance to carbapenems, one of the last resort families of antibiotics. Resistance is usually mediated by carbapenemases coded for by different classes of genes. A prolonged outbreak of carbapenem-resistant K. pneumoniae infections has been recently described in northeastern Ohio. Most strains isolated from patients during this outbreak belong to MLST sequence type 258 (ST258). To understand more about this outbreak two isolates (strains 140 and 677), one of them responsible for a fatal infection, were selected for genome comparison analyses. Whole genome map and sequence comparisons demonstrated that both strains are highly related showing 99% average nucleotide identity. However, the genomes differ at the so-called high heterogeneity zone (HHZ) and other minor regions. This study identifies the potential value of the HHZ as a potential marker for K. pneumoniae clinical and epidemiological studies.
Fil: Ramirez, Maria Soledad. California State University Fullerton; Estados Unidos
Fil: Xie, Gang. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Traglia, German Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Johnson, Shannon L.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Davenport, Karen W.. Los Alamos National High Magnetic Field Laboratory; Estados Unidos
Fil: Van Duin, David. University of North Carolina; Estados Unidos
Fil: Ramazani, Azam. California State University Fullerton; Estados Unidos. University of Oxford; Reino Unido
Fil: Perez, Federico. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos
Fil: Jacobs, Michael R.. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Sherratt, David J.. University of Oxford; Reino Unido
Fil: Bonomo, Robert A.. Louis Stokes Cleveland Department of Veterans Affairs Medical Center; Estados Unidos. Case Western Reserve University School of Medicine; Estados Unidos
Fil: Chain, Patrick S. G.. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Tolmasky, Marcelo E.. California State University Fullerton; Estados Unidos
Subject
Whole genome mapping
Optical map
ST-258
Carbapenem
KPC
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/47880