Immunobiotic Lactobacillus jensenii Elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling...

Authors
Shimazu, Tomoyuki; Villena, Julio Cesar; Tohno, Masanori; Fujie, Hitomi; Hosoya, Shoichi; Shimosato, Takeshi; Aso, Hisashi; Suda, Yoshihito; Kawai, Yasushi; Saito, Tadao; Makino, Seiya; Ikegami, Shuji; Itoh, Hiroyuki; Kitazawa, Haruki
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
The effect of Lactobacillus jensenii TL2937 on the inflammatory immune response triggered by enterotoxigenic Escherichia coli (ETEC) and lipopolysaccharide (LPS) in a porcine intestinal epitheliocyte cell line (PIE cells) was evaluated. Challenges with ETEC or LPS elicited toll-like receptor (TLR)-4-mediated inflammatory responses in cultured PIE cells, indicating that our cell line may be useful for studying inflammation in the guts of weaning piglets. In addition, we demonstrated that L. jensenii TL2937 attenuated the expression of pro-inflammatory cytokines and chemokines caused by ETEC or LPS challenge by downregulating TLR4-dependent nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) activation. Furthermore, we demonstrated that L. jensenii TL2937 stimulation of PIE cells upregulated three negative regulators of TLRs: A20, Bcl-3 and MPK-1, deepening the understanding of an immunobiotic mechanism of action. L. jensenii TL2937-mediated induction of negative regulators of TLRs would have a substantial physiological impact on homeostasis in PIE cells because excessive TLR inflammatory signaling would be downregulated. These results indicated that PIE cells can be used to study the mechanisms involved in the protective activity of immunobiotics against intestinal inflammatory damage and may provide useful information for the development of new immunologically functional feeds that help to prevent inflammatory intestinal disorders, including weaning-associated intestinal inflammation.
Fil: Shimazu, Tomoyuki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Villena, Julio Cesar. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Tohno, Masanori. National Institute Of Livestock And Grassland Science; Japón
Fil: Fujie, Hitomi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón. Josai University. Graduate School of Pharmaceutical Science. Department of Nutritional Physiology; Japón
Fil: Hosoya, Shoichi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Shimosato, Takeshi. Shinshu University. Fiber-Nanotech Young Researcher Empowerment Center; Japón
Fil: Aso, Hisashi. Tohoku University. Graduate School of Agricultural Science. Cell Biology Laboratory; Japón
Fil: Suda, Yoshihito. Miyagi University. Department of Food, Agriculture and Environmental Science; Japón
Fil: Kawai, Yasushi. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Saito, Tadao. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Fil: Makino, Seiya. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Ikegami, Shuji. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Itoh, Hiroyuki. Meiji Dairies Corporation. Division of Research and Development; Japón
Fil: Kitazawa, Haruki. Tohoku University. Graduate School of Agricultural Science. Food Immunology Group; Japón
Subject
LACTOBACILLUS JENSENII TL2937
PORCINE INTESTINAL EPITHELIAL CELLS
TOLL-LIKE RECEPTOR 4
ANTI-INFLAMMATORY ACTIVITY
Tecnologías que involucran la identificación de ADN, proteínas y enzimas, y cómo influyen en el conjunto de enfermedades y mantenimiento del bienestar
Biotecnología de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/16996