Adenosine reagentless electrochemical aptasensor using a phosphorothioate immobilization strategy

Authors
Ceretti, Helena; Ponce, Belén; Ramírez, Silvana A.; Montserrat, Javier Marcelo
Publication Year
2010
Language
English
Format
article
Status
Published version
Description
This work deals with the characterization of a phosphorothioate anchoring strategy for aptamer molecules linked to gold, in the context of electrochemical sensors, using adenosine aptamer as model system. Surface density of immobilized phosphorothioate oligonucleotide sequences has been explored for a range of oligonucleotide concentrations (0.055 -55 μM), finding a consequent variation of molecular surface density (3.5×1011 -2.8 ×1013 molecules/cm2). Most suitable aptamer concentration for adenosine recognition was also explored and found to be around 5.5 μM. As proof of concept of phosphorothioate strategy, electrochemical response to adenosine concentration was measured using a ferrocene-labeled oligonucleotide sequence, and phosphorothioate anchoring thermal stability was compared to thiol immobilization. © 2010 Wiley-VCH Verlag GmbH&Co. KGaA, Weinheim.
Fil: Ceretti, Helena. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ponce, Belén. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Ramírez, Silvana A.. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Fil: Montserrat, Javier Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad Nacional de General Sarmiento. Instituto de Ciencias; Argentina
Subject
ADENOSINE
APTAMERS
ELECTROCHEMICAL
GOLD
PHOSPHOROTHIOATE
Otras Ciencias Químicas
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/53641