Evaluation of inflammatory biomarkers associated with oxidative stress and histological assessment of magnetic therapy on experimental myopathy in rats

Authors
Vignola, María Belén; Dávila, Soledad; Cremonezzi, David; Simes, Juan Carlos; Palma, José A.; Campana, Vilma
Publication Year
2012
Language
English
Format
article
Status
Published version
Description
The effect of pulsed electromagnetic field (PEMF) therapy, also called magnetic therapy, upon inflammatory biomarkers associated with oxidative stress plasma fibrinogen, nitric oxide (NO), L-citrulline, carbonyl groups, and superoxide dismutase (SOD) was evaluated through histological assessment, in rats with experimental myopathy. The groups studied were: (A) control (intact rats that received PEMF sham exposures); (B) rats with myopathy and sacrificed 24 h later; (C) rats with myopathy; (D) rats with myopathy and treated with PEMF; and (E) intact rats treated with PEMF. Groups A, C, D, and E were sacrificed 8 days later. Myopathy was induced by injecting 50 ml of 1% carrageenan l (type IV) once subplantar. Treatment was carried out with PEMF emitting equipment with two flat solenoid disks for 8 consecutive days in groups D and E, at 20 mT and 50 Hz for 30 min/day/rat. The biomarkers were determined by spectrophotometry. The muscles (5/8) were stained with Hematoxylin-Eosin and examined by optic microscopy. Quantitative variables were statistically analyzed by the Fisher test, and categorical applying Pearson?s Chi Squared test at p , 0.05 for all cases. In Groups B and C, the biomarkers were significantly increased compared to A, D, and E groups: fibrinogen (p , 0.001); NO, L-citrulline and carbonyl groups (p , 0.05); SOD (p , 0.01) as well as the percentage of area with inflammatory infiltration (p , 0.001). PEMF caused decreased levels of fibrinogen, L-citrulline, NO, SOD, and carbonyl groups and significant muscle recovery in rats with experimental myopathies.
Fil: Vignola, María Belén. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Dávila, Soledad. Universidad Nacional de La Rioja; Argentina
Fil: Cremonezzi, David. Universidad Nacional de La Rioja; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Simes, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Palma, José A.. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina
Fil: Campana, Vilma. Universidad Nacional de Córdoba. Facultad de Medicina. Departamento de Fisiología Humana y Física Biomedica. Cátedra de Física Biomedica; Argentina. Universidad Nacional de La Rioja; Argentina
Subject
Experimental Myopathy
Inflammatory Biomarkers
Magnetotherapy
Oxidative Stress
Pemf
Pulsed Electromagnetic Field Therapy
Patología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/84092