Simple and easy method for the determination of fungal growth and decolourative capacity in solid media

Authors
Yonni, Fernando; Moreira, María Teresa; Fasoli, Hector; Grandi, Lucila; Cabral, Daniel
Publication Year
2004
Language
English
Format
article
Status
Published version
Description
A technique was developed for studying the biodegradative ability of white rot fungi in different solid media. This technique enables the gravimetric determination of fungal growth (increase of biomass) and the spectrometric measurement of fungal decolourization ability (both by the determination of the production of the extracellular enzyme manganese-dependent peroxidase (MnP) and by the rate of decolourization of dyes). Bjerkandera sp., strain BOS55, was grown in different solid media. Its growth rate, decolourization of solophenil blue 2BL (azoic dye), neutral red (eurhodin dye), methyl green and crystal violet (triphenylmethane dyes) and the production of MnP were determined. Application of this technique enabled a spectrometric quantification of enzymatic activity. Assays indicate that greater amounts of MnP were present in agar plate cultures of Bjerkandera sp. than in liquid cultures.
Fil: Yonni, Fernando. Escuela Superior Técnica "General Manuel N. Savio". Laboratorio de Química; Argentina
Fil: Moreira, María Teresa. Universidad de Santiago de Compostela; España
Fil: Fasoli, Hector. Escuela Superior Técnica "General Manuel N. Savio". Laboratorio de Química; Argentina
Fil: Grandi, Lucila. Escuela Superior Técnica "General Manuel N. Savio". Laboratorio de Química; Argentina
Fil: Cabral, Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Micología y Botánica. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica; Argentina
Subject
ROT FUNGI
FUNGAL GROWTH
DECOLORATIVE CAPACITY
SOLID-MEDIA
DYE
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/45950