Ultrastructure of the cyst wall of Haematococcus pluvialis (Chlorophyceae): wall development and behaviour during cyst germination

Authors
Damiani, Maria Cecilia; Leonardi, Patricia Ines; Pieroni, Olga Inés; Caceres, Eduardo Jorge
Publication Year
2006
Language
English
Format
article
Status
Published version
Description
The cell wall structure and behaviour of cysts of Argentinian strains of Haematococcus pluvialis were studied during cyst germination and zooid release. The cyst wall is composed of an outer primary wall, a trilaminar sheath, a secondary wall, and a tertiary wall. The primary wall disintegrates as the cyst ages. During germination the trilaminar sheath breaks down, carrying the adjacent secondary wall with it and the extensible tertiary wall accompanies the cyst in its size increase and shape changes. Zooid release subsequently follows as a result of the apical breakage of this third wall. Although the secondary and tertiary walls exhibit the same conformation in mature cysts, the arrangement of their components during their formation is different. This could be the reason why the behaviour of the two walls is different during germination. The chemical characterization carried out in the present research showed that the external trilaminar sheath of the wall is formed by algaenan, while fluorescence labelling suggested the presence of mannose and cellulose in the secondary and tertiary walls. Because of the physical difficulty of breaking cysts during astaxanthin extraction, cyst germination may provide a natural, alternative wall-breaking mechanism.
Fil: Damiani, Maria Cecilia. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leonardi, Patricia Ines. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Pieroni, Olga Inés. Universidad Nacional del Sur. Departamento de Química; Argentina
Fil: Caceres, Eduardo Jorge. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia. Laboratorio de Ficología y Micología; Argentina
Subject
Algaenan, , , , , , ,
Ultrastructure
Astaxanthin
Cell wall
Chlorophyceae
Cyst
Germination
Haematococcus pluvialis
Biotecnología Industrial
Biotecnología Industrial
INGENIERÍAS Y TECNOLOGÍAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/34467