Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability

Authors
Figueroa, Carlos Maria; Feil, Regina; Ishihara, Hirofumi; Watanabe, Mutsumi; Kölling, Katharina; Krause, Ursula; Höhne, Melanie; Encke, Beatrice; Plaxton, William C.; Zeeman, Samuel C.; Li, Zhi; Schulze, Waltraud X.; Hoefgen, Rainer; Stitt, Mark; Lunn, John E.
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Trehalose 6-phosphate (Tre6P) is an essential signal metabolite in plants, linking growth and development to carbon metabolism. The sucrose-Tre6P nexus model postulates that Tre6P acts as both a signal and negative feedback regulator of sucrose levels. To test this model, short-term metabolic responses to induced increases in Tre6P levels were investigated in Arabidopsis thaliana plants expressing the Escherichia coli Tre6P synthase (otsA) under the control of an ethanol-inducible promoter. Increased Tre6P led to a transient drop in sucrose content, post-translational activation of nitrate reductase and phosphoenolpyruvate carboxylase, and increased levels of organic and amino acids. Radio- (14CO2) and stable-isotope (13CO2) labelling experiments showed no change in the rates of photoassimilate export in plants with elevated Tre6P, but increased flux of carbon into organic acids. It is concluded that high Tre6P lowers sucrose levels by stimulating nitrate assimilation and anaplerotic synthesis of organic acids, thereby diverting photoassimilates away from sucrose to generate carbon skeletons and fixed nitrogen for amino acid synthesis. These results are consistent with the sucrose-Tre6P nexus model and implicate Tre6P in coordinating carbon and nitrogen metabolism in plants.
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Ishihara, Hirofumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Watanabe, Mutsumi. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Kölling, Katharina. Institute of Agricultural Sciences; Suiza
Fil: Krause, Ursula. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Höhne, Melanie. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Encke, Beatrice. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Plaxton, William C.. Queens University; Canadá
Fil: Zeeman, Samuel C.. Institute of Agricultural Sciences; Suiza
Fil: Li, Zhi. Universidad de Hohenheim; Alemania
Fil: Schulze, Waltraud X.. Universidad de Hohenheim; Alemania
Fil: Hoefgen, Rainer. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Stitt, Mark. Max Planck Institute of Molecular Plant Physiology; Alemania
Fil: Lunn, John E.. Max Planck Institute of Molecular Plant Physiology; Alemania
Subject
ARABIDOPSIS THALIANA
NITRATE REDUCTASE
PROTEIN PHOSPHORYLATION
PHOSPHOENOLPYRUVATE CARBOXYLASE
SUCROSE
TREHALOSE-6-PHOSPHATE SYNTHASE
UBIQUITINATION
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/45420