Bleomycin-induced trans lipid formation in cell membranes and in liposome models

Authors
Cort, Aysegul; Ozben, Tomris; Sansone, Anna; Barata Vallejo, Sebastian; Chatgilialoglu, Chryssostomos; Ferreri, Carla
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Cell cultures of NTera-2 cells incubated with bleomycin and liposomes as biomimetic models of cell membranes were used for examining some novel aspects of drug-metal induced reactivity with unsaturated lipids under oxidative conditions. In cell cultures, bleomycin was found for the first time to cause the formation of trans fatty acids. The chemical basis of this transformation was ascertained by liposome experiments, using bleomycin-iron complexes in the presence of thiol as a reducing agent that by incubation at 37 °C gave rise to the thiyl radical-catalysed double bond isomerisation of membrane phospholipids. The effect of oxygen and reagent concentrations on the reaction outcome was studied. An interesting scenario of free radical reactivity is proposed, which can be relevant for understanding the role of membrane lipids in antitumoral treatments and drug carrier interaction.
Fil: Cort, Aysegul. Akdeniz University. Faculty of Medicine; Turquía. Consiglio Nazionale delle Ricerche; Italia
Fil: Ozben, Tomris. Akdeniz University. Faculty of Medicine; Turquía
Fil: Sansone, Anna. Consiglio Nazionale delle Ricerche; Italia
Fil: Barata Vallejo, Sebastian. Consiglio Nazionale delle Ricerche; Italia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Orgánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chatgilialoglu, Chryssostomos. Demokritos National Centre For Scientific Research; Grecia
Fil: Ferreri, Carla. Consiglio Nazionale delle Ricerche; Italia
Subject
Biomimetics
Fatty acids
Free radicals
Membrane lipids
Otras Ciencias Químicas
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/37421