Identification of selected CITES-protected Araucariaceae using DART TOFMS

Authors
Evans, Philip D.; Mundo, Ignacio Alberto; Wiemann, Michael C.; Chavarria, Gabriela D.; McClure, Pamela J.; Voin, Doina; Espinoza, Edgard O.
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Determining the species source of logs and planks suspected of being Araucaria araucana (Molina) K.Koch (CITES Appendix I) using traditional wood anatomy has been difficult, because its anatomical features are not diagnostic. Additionally, anatomical studies of Araucaria angustifolia (Bertol.) Kuntze, Araucaria heterophylla (Salisb.) Franco, Agathis australis (D.Don) Lindl., and Wollemia nobilis W.G.Jones, K.D.Hill & J.M.Allen have reported that these taxa have similar and indistinguishable anatomical characters from A. araucana. Transnational shipments of illegal timber obscure their geographic provenance, and therefore identification using wood anatomy alone is insufficient in a criminal proceeding. In this study we examine the macroscopic appearance of selected members of the Araucariaceae and investigate whether analysis of heartwood chemotypes using Direct Analysis in Real Time (DART) Time-of-Flight Mass Spectrometry (TOFMS) is useful for making species determinations. DART TOFMS data were collected from 5 species (n =75 spectra). The spectra were analyzsed statistically using supervised and unsupervised classification algorithms. Results indicate that A. araucana can be distinguished from the look-alike taxa. Another statistical inference of the data suggests that Wollemia nobilis is more similar and within the same clade as Agathis australis. We conclude that DART TOFMS spectra can help in making species determination of the Araucariaceae even when the geographic provenance is unknown.
Fil: Evans, Philip D.. University of British Columbia; Canadá
Fil: Mundo, Ignacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
Fil: Wiemann, Michael C.. Center For Wood Anatomy Research; Estados Unidos
Fil: Chavarria, Gabriela D.. United States Fish And Wildlife Service; Estados Unidos
Fil: McClure, Pamela J.. United States Fish And Wildlife Service; Estados Unidos
Fil: Voin, Doina. United States Fish And Wildlife Service; Estados Unidos
Fil: Espinoza, Edgard O.. United States Fish And Wildlife Service; Estados Unidos
Subject
AGATHIS
ARAUCARIA
FORENSICS
ILLEGAL LOGGING
MULTIVARIATE STATISTICS
WOLLEMIA
Meteorología y Ciencias Atmosféricas
Ciencias de la Tierra y relacionadas con el Medio Ambiente
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/63458