Unlipidated Outer Membrane Protein Omp16 (U-Omp16) from Brucella spp. as Nasal Adjuvant Induces a Th1 Immune Response and Modulates the Th2 Allergic Response to Cow’s Milk Proteins...

Authors
Ibañez, Andres Esteban; Smaldini, Paola Lorena; Coria, Lorena; Delpino, María Victoria; Pacífico, Lucila G.; Oliveira, Sergio C.; Risso, Gabriela Sofía; Pasquevich, Karina Alejandra; Fossati, Carlos Alberto; Giambartolomei, Guillermo Hernan; Docena, Guillermo H.; Cassataro, Juliana
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
The discovery of novel mucosal adjuvants will help to develop new formulations to control infectious and allergic diseases. In this work we demonstrate that U-Omp16 from Brucella spp. delivered by the nasal route (i.n.) induced an inflammatory immune response in bronchoalveolar lavage (BAL) and lung tissues. Nasal co-administration of UOmp16 with the model antigen (Ag) ovalbumin (OVA) increased the amount of Ag in lung tissues and induced OVAspecific systemic IgG and T helper (Th) 1 immune responses. The usefulness of U-Omp16 was also assessed in a
mouse model of food allergy. U-Omp16 i.n. administration during sensitization ameliorated the hypersensitivity responses of sensitized mice upon oral exposure to Cow’s Milk Protein (CMP), decreased clinical signs, reduced anti-CMP IgE serum antibodies and modulated the Th2 response in favor of Th1 immunity. Thus, U-Omp16 could be used as a broad Th1 mucosal adjuvant for different Ag formulations.
Fil: Ibañez, Andres Esteban. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Smaldini, Paola Lorena. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune;
Fil: Coria, Lorena. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Delpino, María Victoria. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Pacifico, Lucila G.. Department of Biochemistry and Immunology. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte. Minas Gerais; Brazil;
Fil: Oliveira, Sergio C.. Department of Biochemistry and Immunology. Institute of Biological Sciences. Federal University of Minas Gerais. Belo Horizonte. Minas Gerais; Brazil;
Fil: Risso, Gabriela Sofía. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Pasquevich, Karina Alejandra. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Carlos A Fossati. Consejo Nacional de Invest.cientif.y Tecnicas. Oficina de Coordinacion Administrativa Houssay. Instituto de Est.de la Inmunidad Humoral Prof.r.a.margni;
Fil: Giambartolomei, Guillermo Hernan. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Fil: Docena, Guillermo H.. Universidad Nacional de la Plata. Facultad de Cs.exactas. Departamento de Cs.biologicas. Laboratorio de Invest.del Sistema Inmune;
Fil: Cassataro, Juliana. Universidad de Buenos Aires. Facultad de Medicina. Htal.de Clinicas Gral.san Martin. Laboratorio de Inmunogenetica;
Subject
ADYUVANT
OMP16
BRUCELLA
VACCINE
Ciencias Naturales y Exactas
Ciencias Biológicas
Biología Celular, Microbiología
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/506