In vitro penetration of the antibiotic fosfomycin in swine intestinal cells (IPEC J2)

Authors
Pérez, Denisa Soledad; Martínez, Guadalupe; Soraci, Alejandro Luis; Tapia, Maria Ofelia
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Fosfomycin is an antibiotic widely used for the treatment and prevention of swine infections caused by resistant bacteria. The aim of this research was to study the penetration of fosfomycin in an in vitro model of intestinal cells (IPEC-J2 cells). Cells cultures were subjected to 580 μg/mL of calcium fosfomycin. Intracellular concentrations of the antibiotic were analyzed by HPLC MS/MS and they ranged from 23.48 to 45.81 μg/mL (Tmax: 4 h). Fosfomycin concentrations exceeded the MIC90 for the most important pathogens in swine intestinal infections (Escherichia coli: 0.50 μg/mL, Salmonella enterica subsp. Enterica: 4 μg/mL). Therefore, it is apparent that fosfomycin is an alternative for the treatment of intestinal infections in pigs
Fil: Pérez, Denisa Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Martínez, Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Soraci, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Tapia, Maria Ofelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Subject
Fosfomycin
In vitro
Penetration, IPEC
Intestinal infections, pigs
Ciencias Veterinarias
Ciencias Veterinarias
CIENCIAS AGRÍCOLAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/4689