Evaluation of the protection exerted by Pisum sativum Ferredoxin-NADP(H) Reductase against injury induced by hypothermia on Cos-7 cells

Authors
Pucci Molineris, Melisa Eliana; Di Venanzio, Gisela Andrea; Mamprin, María Eugenia; Mediavilla, Maria Gabriela
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Hypothermia is employed as a method to diminish metabolism rates and preserve tissues and cells. However,low temperatures constitute a stress that produces biochemical changes whose extension depends on the duration and degree of cold exposure and is manifested when physiological temperature is restored. For many cellular types, cold induces an oxidative stress that is dependent on the elevation of intracellular iron, damages macromolecules, and is prevented by the addition of iron chelators. Pisum sativum Ferredoxin-NADP(H) Reductase (FNR) has been implicated in protection from injury mediated by intracellular iron increase and successfully used to reduce oxidative damage on bacterial, plant and mammalian systems. In this work, FNR was expressed in Cos-7 cells; then, they were submitted to cold incubation and iron overload to ascertain whether this enzyme was capable of diminishing the harm produced by these challenges. Contrary to expected, FNR was not protective and even exacerbated the damage under certain circumstances. It was also found that the injury induced by hypothermia in Cos-7 cells presented both iron-dependent and iron-independent components of damage when cells were actively dividing but only iron-independent component when cells were in an arrested state. This is in agreement with previous findings which showed that iron-dependent damage is also an energy-dependent process.
Fil: Pucci Molineris, Melisa Eliana. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiologia Cli­nica y Aplicada; Argentina
Fil: Di Venanzio, Gisela Andrea. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiología Clínica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Mamprin, María Eugenia. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiologia Cli­nica y Aplicada; Argentina
Fil: Mediavilla, Maria Gabriela. Universidad Nacional de Rosario. Facultad de Humanidades y Artes. Secretaria de Ciencia y Tecnica. Centro Binacional (Argentina- Italia) de Investigaciones en Criobiologia Cli­nica y Aplicada; Argentina
Subject
Hypothermia
Mammalian cells
Plant ferredoxin reductase
Oxidative stress
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/5233