Ethanol-induced oxidative stress and acetaldehyde formation in rat mammary tissue: Potential factors involved in alcohol drinking promotion of breast cancer

Authors
Castro, Gerardo Daniel; Rodríguez de Castro, Carmen; Maciel, Maria Eugenia; Fanelli, Silvia Laura; Ferreyra, Elida Vilma; Diaz Gomez, Maria Isabel; Castro, Jose Alberto
Publication Year
2005
Language
English
Format
article
Status
Published version
Description
Recent studies from our laboratory provided evidence that part of the carcinogenic effects of ethanol consumption might be related to its in situ metabolism at cytosolic and microsomal levels, to the mutagen acetaldehyde and to hydroxyl and 1-hydroxyethyl radicals. In this work, we report on our experiments where Sprague–Dawley female rats were exposed to the standard Lieber & De Carli diet for 28 days. We observed: the induction of the (xanthineoxidoreductase mediated) cytosolic and microsomal (lipoxygenase mediated) pathways of ethanol metabolism; promotion of oxidative stress as shown by increased formation of lipid hydroperoxides; delay in the t-butylhydroperoxide induced chemiluminiscence, and a significant decrease in protein sulfhydryls. In addition, the epithelial cells showed ultrastructural alterations consisting of markedly irregular nuclei, with frequent invaginations at the level of the nuclear envelope, condensation of chromatin around the inner nuclear membrane, and marked dilatation of the nuclear pores showing filamentous material exiting to the cytoplasm. In conclusion, the presence in mammary epithelial cells of cytosolic and microsomal pathways of ethanol bioactivation to carcinogenic and to tumorigenic metabolites might play a role in alcohol promotion of breast cancer.
Fil: Castro, Gerardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Rodríguez de Castro, Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Maciel, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Fanelli, Silvia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Limnología "Dr. Raúl A. Ringuelet". Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Limnología; Argentina
Fil: Ferreyra, Elida Vilma. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Diaz Gomez, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Fil: Castro, Jose Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Gp.citefa - Centro de Investigaciones Toxicológicas (i); Argentina
Subject
ethanol
oxidative stress
acetaldehyde
Cancer
Breast
Bioquímica y Biología Molecular
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/27591