A BK (Slo1) channel journey from molecule to physiology

Authors
Contreras, Gustavo F.; Castillo, Karen Noel; Enrique, Nicolás Jorge; Carrasquel Ursulaez, William; Castillo, Juan Pablo; Milesi, Verónica; Neely, Allan; Alvarez, Osvaldo; Ferreira, Gonzalo; Gonzáez, Carlos; Latorre, Ramón
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Calcium and voltage-activated potassium (BK) channels are key actors in cell physiology, both in neuronal and nonneuronal cells and tissues. Through negative feedback between intracellular Ca2+ and membrane voltage, BK channels provide a damping mechanism for excitatory signals. Molecular modulation of these channels by alternative splicing, auxiliary subunits and post-translational modifications showed that these channels are subjected to many mechanisms that add diversity to the BK channel α subunit gene. This complexity of interactions modulates BK channel gating, modifying the energetic barrier of voltage sensor domain activation and channel opening. Regions for voltage as well as Ca2+ sensitivity have been identified, and the crystal structure generated by the 2 RCK domains contained in the C-terminal of the channel has been described. The linkage of these channels to many intracellular metabolites and pathways, as well as their modulation by extracellular natural agents, has been found to be relevant in many physiological processes. This review includes the hallmarks of BK channel biophysics and its physiological impact on specific cells and tissues, highlighting its relationship with auxiliary subunit expression.
Fil: Contreras, Gustavo F.. Universidad de Valparaíso; Chile
Fil: Castillo, Karen Noel. Universidad de Valparaíso; Chile
Fil: Enrique, Nicolás Jorge. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Grupo de Investigación en Fisiología Vascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carrasquel Ursulaez, William. Universidad de Valparaíso; Chile
Fil: Castillo, Juan Pablo. Universidad de Valparaíso; Chile. Universidad de Chile; Chile
Fil: Milesi, Verónica. Universidad Nacional de la Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Grupo de Investigación en Fisiología Vascular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Neely, Allan. Universidad de Valparaíso; Chile
Fil: Alvarez, Osvaldo. Universidad de Chile; Chile
Fil: Ferreira, Gonzalo. Universidad de la República; Uruguay
Fil: Gonzáez, Carlos. Universidad de Valparaíso; Chile
Fil: Latorre, Ramón. Universidad de Valparaíso; Chile
Subject
BK channels
Slo1
Auxiliary subunits
Voltage sensor
Intracellular Ca2+
Smooth muscle
Diseases
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/23668