Epithelial Ovarian Cancer Diagnosis of SecondHarmonic Generation Images: A Semiautomatic Collagen Fibers Quantification Protocol

Authors
Zeitoune, Angel Alberto; Luna, Johana S. J.; Sanchez Salas, Kynthia; Erbes, Luciana Ariadna; Cesar, Carlos L.; Andrade, Liliana A. L. A.; Carvahlo, Hernades F.; Bottcher Luiz, Fátima; Casco, Victor Hugo; Adur, Javier Fernando
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
A vast number of human pathologic conditions are directly or indirectly related to tissular collagen structure remodeling. The nonlinear optical microscopy second-harmonic generation has become a powerful tool for imaging biological tissues with anisotropic hyperpolarized structures, such as collagen. During the past years, several quantification methods to analyze and evaluate these images have been developed. However, automated or semiautomated solutions are necessary to ensure objectivity and reproducibility of such analysis. This work describes automation and improvement methods for calculating the anisotropy (using fast Fourier transform analysis and the gray-level co-occurrence matrix). These were applied to analyze biopsy samples of human ovarian epithelial cancer at different stages of malignancy (mucinous, serous, mixed, and endometrial subtypes). The semiautomation procedure enabled us to design a diagnostic protocol that recognizes between healthy and pathologic tissues, as well as between different tumor types.
Fil: Zeitoune, Angel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Luna, Johana S. J.. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Sanchez Salas, Kynthia. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Erbes, Luciana Ariadna. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina
Fil: Cesar, Carlos L.. Universidade Federal do Ceará; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil
Fil: Andrade, Liliana A. L. A.. Universidade Estadual de Campinas; Brasil
Fil: Carvahlo, Hernades F.. Universidade Estadual de Campinas; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil
Fil: Bottcher Luiz, Fátima. Universidade Estadual de Campinas; Brasil. National Institute of Science and Technology on Photonics Applied to Cell Biology; Brasil
Fil: Casco, Victor Hugo. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Fil: Adur, Javier Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Entre Ríos. Universidad Nacional de Entre Ríos. Centro de Investigaciones y Transferencia de Entre Ríos; Argentina. Universidad Nacional de Entre Ríos. Facultad de Ingeniería; Argentina
Subject
FFT
GLCM
SHG MICROSCOPY
HUMAN CANCER OVARY
DIAGNOSTIC PROTOCOL
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/42377