Characterization and expression analysis of Somatic Embryogenesis Receptor Kinase (SERK) genes in sexual and apomictic Paspalum notatum

Authors
Podio, Maricel; Felitti, Silvina Andrea; Siena, Lorena Adeli; Delgado Benarroch, Luciana; Mancini, Micaela; Seijo, Jose Guillermo; Gonzalez, Ana María; Pessino, Silvina Claudia; Ortiz, Juan Pablo Amelio
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
The Somatic Embryogenesis Receptor Kinase (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression patterns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indicated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apomictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Fil: Siena, Lorena Adeli. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;
Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;
Fil: Gonzalez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina; Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;
Subject
PASPALUM NOTATUM
SERK
APOMIXIS
APOSPORY
QRT-PCR
IN SITU HYBRIDIZATION
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/2226