Differential Role of the T6SS in Acinetobacter baumannii Virulence

Authors
Repizo, Guillermo Daniel; Gagné, Stéphanie; Foucault Grunenwald, Marie Laure; Borges, Vitor; Charpentier, Xavier; Limansky, Adriana Sara; Gomes, João Paulo; Viale, Alejandro Miguel; Salcedo, Suzana P.
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Gram-negative bacteria, such as Acinetobacter baumannii, are an increasing burden in hospitals worldwide with an alarming spread of multi-drug resistant (MDR) strains. Herein, we compared a type strain (ATCC17978), a non-clinical isolate (DSM30011) and MDR strains of A. baumannii implicated in hospital outbreaks (Ab242, Ab244 and Ab825), revealing distinct patterns of type VI secretion system (T6SS) functionality. The T6SS genomic locus is present and was actively transcribed in all of the above strains. However, only the A. baumannii DSM30011 strain was capable of killing Escherichia coli in a T6SS-dependent manner, unlike the clinical isolates, which failed to display an active T6SS in vitro. In addition, DSM30011 was able to outcompete ATCC17978 as well as Pseudomonas aeruginosa and Klebsiella pneumoniae, bacterial pathogens relevant in mixed nosocomial infections. Finally, we found that the T6SS of DSM30011 is required for host colonization of the model organism Galleria mellonella suggesting that this system could play an important role in A. baumannii virulence in a strain-specific manner.
Fil: Repizo, Guillermo Daniel. Université Claude Bernard Lyon 1; Francia
Fil: Gagné, Stéphanie. Université Claude Bernard Lyon 1; Francia
Fil: Foucault Grunenwald, Marie Laure. Université Claude Bernard Lyon 1; Francia
Fil: Borges, Vitor. National Institute of Health; Portugal
Fil: Charpentier, Xavier. Université Claude Bernard Lyon 1; Francia
Fil: Limansky, Adriana Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gomes, João Paulo. National Institute of Health; Portugal
Fil: Viale, Alejandro Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Salcedo, Suzana P.. Université Claude Bernard Lyon 1; Francia
Subject
Acinetobacter baumannii
Virulence
T6SS
Bacterial dueling
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/21530