Association of the time course of pulmonary arterial hypertension with changes in oxidative stress in the left ventricle

Authors
Leichsenring Silva, Fabiano; Tavares, Angela Maria Vicente; Mosele, Francisca; Berger, Bruno; Llesuy, Susana Francisca; Belló Klein, Adriane
Publication Year
2011
Language
English
Format
article
Status
Published version
Description
1. This study investigates the time course of pulmonary arterial hypertension (PAH) due to monocrotaline (MCT) and its association with cardiac function and oxidative stress markers in the left ventricle (LV). 2. Male Wistar rats were divided into six groups: 7days, 21days, and 31days for both control and MCT groups. Following echocardiographic analysis, the heart was removed. The LV was separated and homogenized to analyze oxidized-to-total glutathione ratio and thioredoxin reductase (TrxR) activity as well as hydrogen peroxide (H 2O 2) and ascorbic acid levels. 3. There was significant (P<0.01) cardiac and right ventricle (RV) hypertrophy and pulmonary congestion in the MCT 21day and 31day groups. Echocardiography showed a change in the flow wave of the pulmonary artery at 21days after MCT treatment. There was an increase in the LV ejection time (P<0.05) at 31days after MCT. The LV H 2O 2 concentration was increased (P<0.05) in the MCT 21day and MCT 31day groups compared with controls. There was a reduction (P<0.05) in the LV ascorbic acid concentration and an increase (P<0.05) in TrxR activity in the MCT 31day rats. 4. Our findings showed RV changes due to pulmonary hypertension at 21days after MCT injection. There was a correlation between the degree of dysfunction and the morphometry of the heart chambers, along with impairment of the antioxidant/pro-oxidant balance in the LV 31days after the beginning of the protocol. This study suggests that LV changes follow RV dysfunction subsequent to pulmonary hypertension.
Fil: Leichsenring Silva, Fabiano. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Tavares, Angela Maria Vicente. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mosele, Francisca. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Berger, Bruno. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Llesuy, Susana Francisca. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Belló Klein, Adriane. Universidade Federal do Rio Grande do Sul; Brasil
Subject
ASCORBIC ACID
COR PULMONALE
GLUTATHIONE
MONOCROTALINE
RIGHT HEART FAILURE
THIOREDOXIN REDUCTASE
Otras Medicina Básica
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/67467