A novel solid phase extraction Ultra high performance liquid chromatography tandem mass spectrometry method for the quantification of ochratoxin A in red wines

Authors
Marino Repizo, Leonardo; Kero, Frank; Vandell, Victor; Senior, Adam; Sanz Ferramola, Maria Isabel; Cerutti, Estela Soledad; Raba, Julio
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
A novel and advanced technology on solid phase extraction column prior to liquid chromatography coupled to tandem mass spectrometry has been used for the determination of ochratoxin A in red wine samples. Due to the need of a reliable and rugged method according to current regulations and with the aim of minimize heuristic efforts associated with analytical method development, the statistical design of experiment was employed. On other hand, the method validation according to European Commission 2002/657/EC was achieved. The values obtained for decision limit (CCa), detection capability (CCb), limits of detection and quantification were 0.07 lg L1, 0.14 lg L1, 0.13 lg L1 and 0.41 lg L1, respectively. The recoveries values were ranged from 95.7% to 107.2%. These values were compatible with the 2.0 lg L1 maximum allowable concentration limit established by different international regulations.
Fil: Marino Repizo, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Kero, Frank. Biotage; Estados Unidos
Fil: Vandell, Victor. Biotage; Estados Unidos
Fil: Senior, Adam. Biotage; Estados Unidos
Fil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Cerutti, Estela Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Subject
Ochratoxin A
Red Wine
Solid Phase Extraction
UPLC-MS/MS
Química Analítica
Ciencias Químicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/5761