Abiotic conditions leading to FUM gene expression and fumonisin accumulation by Fusarium proliferatum strains grown on a wheat-based substrate

Authors
Cendoya, Eugenia; Pinson-Gadais, Laetitia; Farnochi, Maria Cecilia; Ramirez, Maria Laura; Chéreau, Sylvain; Marcheguay, Giselè; Ducos, Christine; Barreau, Christian; Richard-Forget, Florence
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Fusarium proliferatum produces fumonisins B not only on maize but also on diverse crops including wheat. Using a wheat-based medium, the effects of abiotic factors, temperature and water activity (aW), on growth, fumonisin biosynthesis, and expression of FUM genes were compared for three F. proliferatum strains isolated from durum wheat in Argentina. Although all isolates showed similar profiles of growth, the fumonisin production profiles were slightly different. Regarding FUM gene transcriptional control, both FUM8 and FUM19 expression showed similar behavior in all tested conditions. For both genes, expression at 25 °C correlated with fumonisin production, regardless of the aw conditions. However, at 15 °C, these two genes were as highly expressed as at 25 °C although the amounts of toxin were very weak, suggesting that the kinetics of fumonisin production was slowed at 15 °C. This study provides useful baseline data on conditions representing a low or a high risk for contamination of wheat kernels with fumonisins.
Fil: Cendoya, Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Pinson-Gadais, Laetitia. Institut National de la Recherche Agronomique; Francia
Fil: Farnochi, Maria Cecilia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina
Fil: Ramirez, Maria Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología. Cátedra de Micología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Chéreau, Sylvain. Institut National de la Recherche Agronomique; Francia
Fil: Marcheguay, Giselè. Institut National de la Recherche Agronomique; Francia
Fil: Ducos, Christine. Institut National de la Recherche Agronomique; Francia
Fil: Barreau, Christian. Institut National de la Recherche Agronomique; Francia
Fil: Richard-Forget, Florence. Institut National de la Recherche Agronomique; Francia
Subject
ABIOTIC STRESS
FUM GENE
FUMONISIN
FUSARIUM PROLIFERATUM
GENE EXPRESSION
WHEAT
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/76266