Both Volatiles and Cuticular Plant Compounds Determine Oviposition of the Willow Sawfly Nematus oligospilus on Leaves of Salix spp. (Salicaceae)

Authors
Braccini, Celina L.; Vega, Andrea Susana; Coll Araoz, Maria Victoria; Teal, Peter E.; Cerrillo, Teresa; Zavala, Jorge Alberto; Fernandez, Patricia Carina
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
Plant volatile organic compounds play a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, contact cues determine host acceptance. Although the willow sawfly Nematus oligospilus (Hymenoptera: Tenthredinidae) can differentiate among willow genotypes, no knowledge is available on the cues used by this insect to seek and accept the host plant. In this study, we recorded behavioral orientation in a Y-tube olfactometer of willow sawfly females to volatiles of the highly preferred genotype Salix nigra and the non-preferred genotype S. viminalis. The volatiles released by undamaged willows of each genotype were analyzed by coupled gas chromatography-mass spectrometry. Contact cues were evaluated first by oviposition preference bioassays after selective leaf wax removal, and then by studying the micromorphology of abaxial and adaxial leaf surfaces and their chemical composition. Willow sawfly females oriented preferentially to S. nigra volatiles, which contained more than 3 times theamount of volatiles than that collected from S. viminalis. Analysis of volatiles showed significant differences in amounts of (Z) and (E)-β-ocimene, undecane, decanal, and β-caryophyllene. The adaxial leaf surface of S. nigra was less preferred after wax removal, suggesting a role of cuticular waxes for oviposition acceptance. No differences were found among the micromorphology of leaf surfaces between preferred and non-preferred genotypes. The chemical analysis of cuticular waxes showed that the abaxial leaf surface of S. viminalis, which is completely avoided for oviposition, possessed 97 % of alkanes. The accepted leaf surfaces contained a more diverse wax profile including alcohols, acids, and esters. Thus, non-alkane wax compounds might be related to oviposition. In sum, our study suggests that several cues act in concert to provide oviposition cues for the sawfly N. oligospilus: females are attracted to volatiles from a distance, and once alighting on the plant, they seek specific chemical contact cues in order to lay eggs.
Fil: Braccini, Celina L.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Recursos Biológicos; Argentina
Fil: Vega, Andrea Susana. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Coll Araoz, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Teal, Peter E.. United States Department of Agriculture; Argentina
Fil: Cerrillo, Teresa. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria Delta del Paraná; Argentina
Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernandez, Patricia Carina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Norte. Estación Experimental Agropecuaria Delta del Paraná; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
OVIPOSITION CUES
TENTHREDINIDAE
INSECT-PLANT INTERACTION
HOST PLANT LOCATION
VOLATILES
CUTICULAR WAX
OLFACTOMETER
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/42067