Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

Authors
Bencke Malato, Marta; Cabreira, Caroline; Wiebke Strohm, Beatriz; Bücker Neto, Lauro; Mancini, Estefania; Osorio, Marina B.; Homrich, Milena S.; Turchetto Zolet, Andreia C.; De Carvalho, Mayra C.C.G.; Stolf, Renata; Weber, Ricardo L.M.; Westergaard, Gastón; Castagnaro, Atilio Pedro; Abdelnoor, Ricardo V.; Marcelino Guimarães, Francismar C.; Margis Pinheiro, Márcia; Bodanese Zanettini, Maria H.
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina
Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina
Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina
Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil
Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
Subject
ASIAN SOYBEAN RUST
FUNCTIONAL ANALYSIS
FUNGUS RESISTANCE
GENETIC TRANSFORMATION
GLYCINE MAX
TRANSCRIPTION FACTORS
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/36572