Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina

Authors
Debiaggi, Maria Florencia; Soriano, Silvia Viviana; Pierangeli, Nora Beatriz; Lazzarini, Lorena Evelina; Pianciola, Luis Alfredo; Mazzeo, Melina Leonor; Moguillansky, Sergio; Basualdo Farjat, Juan Angel
Publication Year
2017
Language
English
Format
article
Status
Published version
Description
Human cystic echinococcosis caused by the larval stage of Echinococcus granulosus sensu lato (s.l.) is a highly endemic disease in the province of Neuquén, Patagonia, Argentina. Human infections with E. granulosus sensu stricto (s.s.) G1 and Echinococcus canadensis G6 were reported in Neuquén in previous studies, whereas four genotypes were identified in livestock: G1, G3, G6, and G7. The aim of this study was to identify the genotypes of E. granulosus s.l. isolates from humans of Neuquén province, Patagonia, Argentina, through the 2005–2014 period. Twenty six hydatid cysts were obtained from 21 patients. The most frequent locations were the liver and lungs. Single cysts were observed in 81.0% of patients, and combined infection of liver and lungs was detected in 9.5% of cases. Partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes identified the presence of E. granulosus s.s. G1 (n = 11; 42.3%) including three different partial sequences; E. canadensis G6 (n = 14; 53.8%) and E. canadensis G7 (n = 1; 3.9%). Coinfection with G1 and G7 genotypes was detected in one patient who harbored three liver cysts. Most of the liver cysts corresponded to G1 and G6 genotypes. This study presents the first report in the Americas of a human infection with E. canadensis G7 and the second worldwide report of a coinfection with two different species and genotypes of E. granulosus s.l in humans. The molecular diversity of this parasite should be considered to redesign or improve the control program strategies in endemic regions.
Fil: Debiaggi, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina. Universidad Nacional del Comahue. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soriano, Silvia Viviana. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Pierangeli, Nora Beatriz. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Lazzarini, Lorena Evelina. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Pianciola, Luis Alfredo. Provinica de de Neuquén. Subsecretaría de Salud; Argentina
Fil: Mazzeo, Melina Leonor. Provinica de de Neuquén. Subsecretaría de Salud; Argentina
Fil: Moguillansky, Sergio. Universidad Nacional del Comahue. Facultad de Medicina; Argentina
Fil: Basualdo Farjat, Juan Angel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Subject
COINFECTION
ECHINOCOCCUS CANADENSIS
ECHINOCOCCUS GRANULOSUS
G7 GENOTYPE
HUMAN ECHINOCOCCOSIS
Salud Ocupacional
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/79045