Effect of cooling rate on sperm quality of cryopreserved Andalusian donkey spermatozoa

Authors
Demyda-Peyrás, Sebastian; Bottrel, M.; Acha, D.; Ortiz, I.; Hidalgo, M.; Carrasco, J.J.; Gómez Arrones, V.; Gósalvez, J.; Dorado, J.
Publication Year
2018
Language
English
Format
article
Status
Published version
Description
The aim of this study was to evaluate the effect of different cooling rates on post-thaw quality of cryopreserved donkey spermatozoa. Eighteen ejaculates from six adult Andalusian donkeys (three ejaculates per donkey) were collected using an artificial vagina. Pooled semen samples (two ejaculates per pool) were divided into three aliquots, and frozen in Gent freezing extender using three different cryopreservation protocols (P): P1 (conventional slow freezing, as control): semen pre-cooled in an Equitainer for 2 h and frozen in liquid nitrogen (LN2) vapour; P2 (controlled pre-freeze cooling rate): semen pre-cooled at a controlled rate for 73 min and frozen in LN2 vapour; and P3 (rapid freezing) semen frozen immediately in LN2 vapour. After thawing at 37 °C for 30 s, semen samples were assessed for motility, morphology, acrosome and plasma membrane integrity; spermatozoa were also tested for DNA integrity. Significant (P < 0.01) differences were found between the cryopreservation protocols for all sperm parameters evaluated, except for DNA integrity. Semen samples frozen using P2 showed significantly (P < 0.01) higher values for sperm motility, morphology, sperm membrane integrity, and acrosome integrity. On the contrary, P3 reduced sperm motility (P < 0.01) and increased the percentage of spermatozoa with damaged plasma membrane (P < 0.001). In our study, we demonstrated that the sperm of Andalusian donkey is particularly sensitive to the cooling rate used before freezing. Furthermore, Andalusian donkey semen can be successfully cryopreserved using controlled cooling rates combined with freezing in LN2 vapour.
Fil: Demyda-Peyrás, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad de Córdoba; España
Fil: Bottrel, M.. Universidad de Córdoba; España
Fil: Acha, D.. Universidad de Córdoba; España
Fil: Ortiz, I.. Universidad de Córdoba; España
Fil: Hidalgo, M.. Universidad de Córdoba; España
Fil: Carrasco, J.J.. Centro de Selección y Reproducción Animal; España
Fil: Gómez Arrones, V.. Centro de Selección y Reproducción Animal; España
Fil: Gósalvez, J.. Universidad Autónoma de Madrid; España
Fil: Dorado, J.. Universidad de Córdoba; España
Subject
COOLING RATE
CRYOPRESERVATION
DONKEY
EQUUS ASINUS
SPERMATOZOA
Otras Biotecnología Agropecuaria
Biotecnología Agropecuaria
CIENCIAS AGRÍCOLAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/82746