Age-related differences in the gastrointestinal microbiota of chinstrap penguins (pygoscelis antarctica)

Authors
Barbosa, Andrés; Balagué, Vanessa; Valera, Francisco; Martínez, Ana; Benzal, Jesús; Motas, Miguel; Diaz, Julia Inés; Mira, Alex; Pedrós Alió, Carlos
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
The gastrointestinal tract microbiota is known to play very important roles in the well being of animals. It is a complex community composed by hundreds of microbial species interacting closely among them and with their host, that is, a microbial ecosystem. The development of high throughput sequencing techniques allows studying the diversity of such communities in a realistic way and considerable work has been carried out in mammals and some birds such as chickens. Wild birds have received less attention and in particular, in the case of penguins, only a few individuals of five species have been examined with molecular techniques. We collected cloacal samples from Chinstrap penguins in the Vapour Col rookery in Deception Island, Antarctica, and carried out pyrosequencing of the V1-V3 region of the 16S rDNA in samples from 53 individuals, 27 adults and 26 chicks. This provided the first description of the Chinstrap penguin gastrointestinal tract microbiota and the most extensive in any penguin species. Firmicutes, Bacteoridetes, Proteobacteria, Fusobacteria, Actinobacteria, and Tenericutes were the main components. There were large differences between chicks and adults. The former had more Firmicutes and the latter more Bacteroidetes and Proteobacteria. In addition, adults had richer and more diverse bacterial communities than chicks. These differences were also observed between parents and their offspring. On the other hand, nests explained differences in bacterial communities only among chicks. We suggest that environmental factors have a higher importance than genetic factors in the microbiota composition of chicks. The results also showed surprisingly large differences in community composition with other Antarctic penguins including the congeneric Adélie and Gentoo penguins.
Fil: Barbosa, Andrés. Consejo Superior de Investigaciones Cientificas. Museo Nacional de Ciencias Naturales; España
Fil: Balagué, Vanessa. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Fil: Valera, Francisco. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Martínez, Ana. Universidad Nacional Autónoma de México; México
Fil: Benzal, Jesús. Consejo Superior de Investigaciones Científicas. Estación Experimental de Zonas Aridas; España
Fil: Motas, Miguel. Universidad de Murcia; España
Fil: Diaz, Julia Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: Mira, Alex. Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana; España
Fil: Pedrós Alió, Carlos. Consejo Superior de Investigaciones Científicas. Instituto de Ciencias del Mar; España
Subject
microbiota
penguin
gastrointestinal tract
pyrsequencing
Diversity
Antarctica
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/48998