Duplex PCR for the diagnosis of Brucella melitensis and its differentiation from the REV-1 vaccine strain

Authors
Alvarez, Lucía Paula; Marcellino, R. B.; Martinez, A.; Robles, C. A.
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Caprine brucellosis is a chronic infectious disease caused by Brucella melitensis, whose main symptoms are abortion in females and orchitis in males. Vaccination by mistake of pregnant goats with the REV-1 vaccine may cause abortions. The aim of this study was to develop a duplex PCR that could identify and differentiate the DNA of B. melitensis field strains from that of the REV-1 vaccine strain. The results showed that the duplex PCR performed on DNA isolated from infected tissues is very convenient as it significantly shortens the time of detection of the etiological agent, yields a reliable result and has an acceptable cost.
Fil: Alvarez, Lucía Paula. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; Argentina
Fil: Marcellino, R. B.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Martinez, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Fil: Robles, C. A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche; Argentina
Subject
Goats
Brucella melitensis
REV-1
Molecular diagnosis
PCR
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/74375