A Neospora caninum vaccine using recombinant proteins fails to prevent foetal infection in pregnant cattle after experimental intravenous challenge

Authors
Hecker, Yanina Paola; Cóceres, Verónica Mabel; Wilkowsky, Silvina Elizabeth; Jaramillo Ortiz, José Manuel; Morrell, Eleonora Lidia; Verna, Andrea Elizabeth; Ganuza, Agustina; Cano, Dora B.; Lischinsky, Lilian; Ángel, Sergio Oscar; Zamorano, Patricia Ines; Odeón, Anselmo Carlos; Leunda, Maria Rosa; Campero, Carlos Manuel; Morein, Bror; Moore, Dadin Prando
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
The aim of the present study was to evaluate the immunogenicity and protective efficacy of rNcSAG1, rNcHSP20, rNcGRA7 recombinant proteins formulated with immune stimulating complexes (ISCOMs) in pregnant heifers against vertical transmission of N. caninum. Twelve pregnant heifers were divided into 3 groups of 4 heifers each, receiving different formulations before mating. Immunogens were administered twice subcutaneously: group A animals were inoculated with three recombinant proteins (rNcSAG1, rNcHSP20, rNcGRA7) formulated with ISCOMs; group B animals received ISCOM-MATRIX (without antigen) and group C received sterile phosphate-buffered saline (PBS) only. The recombinant proteins were expressed in Escherichia coli and purified by nickel resin. All groups were intravenously challenged with the NC-1 strain of Neospora caninum at day 70 of gestation and dams slaughtered at week 17 of the experiment. Heifers from group A developed specific antibodies against rNcSAG1, rNcHSP20 and rNcGRA7 prior to the challenge. Following immunization, an statistically significant increase of antibodies against rNcSAG1 and rNcHSP20 in all animals of group A was detected compared to animals in groups B and C at weeks 5, 13 and 16 (P <0.001). Levels of antibodies against rNcGRA7 were statistical higher in group A animals when compared with groups B and C at weeks 5 and 16 (P > 0.001). There were no differences in IFN-γ production among the experimental groups at any time point (P > 0.05). Transplacental transmission was determined in all fetuses of groups A, B and C by Western Blot, Immunohistochemistry and nested PCR. This work showed that rNcSAG1, rNcHSP20 and rNcGRA7 proteins while immunogenic in cattle failed to prevent the fetal infection in pregnant cattle challenged at Day 70 of gestation.
Fil: Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cóceres, Verónica Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina
Fil: Wilkowsky, Silvina Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Jaramillo Ortiz, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morrell, Eleonora Lidia. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ganuza, Agustina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Cano, Dora B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Lischinsky, Lilian. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ángel, Sergio Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Leunda, Maria Rosa. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Morein, Bror. Uppsala University. Department of Medical Sciences. Infectious Diseases; Suecia
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Subject
Neospora caninum
vaccine
rNcSAG1
rNcHSP20
rNcGRA7
ISCOMs
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/33656