Recombinant protein expression in microbial systems

Authors
Rosano, German Leandro; Ceccarelli, Eduardo Augusto
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
The emergence of recombinant DNA technology during the early 70's set a revolution in molecular biology. This set of techniques was strengthened even further later on with the introduction of the polymerase chain reaction and allowed scientists to explore and understand essential life processes in an easy and straightforward way. It also marked the birth of the modern biotech industry. At that time, it was shown that eukaryotic DNA could be propagated in Escherichia coli (Morrow et al., 1974) and functional products could be synthesized from heterologous genes cloned in bacterial plasmids (Ratzkin and Carbon, 1977; Vapnek et al., 1977). After these successful cases, it was soon realized that the potential applications of these techniques were almost limitless. In fact, US patent 4,237,224 granted to Cohen and Boyer (1980) claimed to commercial ownership of the methodology for cloning virtually all possible DNAs in all possible vectors. While cloning any gene in any given vector is feasible, obtaining a functional product from its expression is not that simple.
Fil: Rosano, German Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Subject
RECOMBINANT PROTEINS
MICROORGANISM
INCLUSION BODIES
FUSION TAGS
ESCHERICHIA COLI
YEAST
FILAMENTOUS FUNGI
MICROALGAE
Bioquímica y Biología Molecular
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/21362