Characterization of Hybrid Bioactive Glass-polyvinyl Alcohol Scaffolds Containing a PTHrP-derived Pentapeptide as Implants for Tissue Engineering Applications

Authors
Coletta, Dante Jesus; Lozano, D.; Rocha Oliveira, A. A.; Mortarino, P.; Bumaguin, G. E.; Vitelli, E.; Vena, Rodrigo; Missana, Liliana Raquel; Jammal, María Victoria; Portal Núñez, S.; Pereira, M.; Esbrit, P.; Feldman, Susana Raquel
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Hybrid foam (BG-PVA) with 50 % Bioactive glass (BG) and 50 % polyvinyl alcohol (PVA) was prepared by sol-gel process to produce scaffolds for bone tissue engineering. The pore structure of hydrated foams was evaluated by 3-D confocal microscopy, confirming 70% porosity and interconnected macroporous network. In this study, we assessed the putative advantage of coating with osteostatin pentapeptide into BG-PVA hybrid scaffolds to improve their bioactivity. In vitro cell culture experiments were performed using mouse pre-osteoblastic MC3T3-E1 cell line. The exposure to osteostatin loaded-BG-PVA scaffolds increase cell proliferation in contrast with the unloaded scaffolds. An in vivo study was selected to implant BG-PVA scaffolds, non-coated (Group A) or coated (Group B) with osteostatin into non critical bone defect at rabbit femur. Both groups showed new compact bone formation on implant surface, with lamellae disposed around a haversian canal forming osteons-like structure. We observed signs of inflammation around the implanted unloaded scaffold at one month, but resolved at 3 months. This early inflammation did not occur in Group B; supporting the notion that osteostatin may act as anti-inflammatory inhibitor. On the other hand, Group B showed increased bone formation, as depicted by many new trabeculae partly mineralized in the implant regenerating area, incipient at 1 month and more evident at 3 months after implantation. PVA/BG hybrid scaffolds present a porous structure suitable to support osteoblast proliferation and differentiation. Our in vitro and in vivo findings indicate that osteostatin coating improves the osteogenic features of these scaffolds.
Fil: Coletta, Dante Jesus. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina;
Fil: Lozano, D.. Universidad Carlos III de Madrid. Instituto de Salud; España;
Fil: Rocha Oliveira, A. A.. Universidade Federal do Minas Gerais; Brasil;
Fil: Mortarino, P.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina;
Fil: Bumaguin, G. E.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina;
Fil: Vitelli, E.. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina;
Fil: Vena, Rodrigo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Rosario. Instituto de Biología Molecular y Celular de Rosario; Argentina;
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina; Universidad Nacional de Tucumán. Facultad de Odontología; Argentina;
Fil: Jammal, María Victoria. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina;
Fil: Portal Núñez, S.. Universidad Carlos III de Madrid. Instituto de Salud; España;
Fil: Pereira, M.. Universidade Federal do Minas Gerais; Brasil;
Fil: Esbrit, P.. Universidad Carlos III de Madrid. Instituto de Salud; España;
Fil: Feldman, Susana Raquel. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Biología Osteoarticular, Ingeniería Tisular y Terapias Emergentes; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
Subject
Bone regeneration,
Hybrid matrix,
Bioactive glass,
Polyvinyl alcohol,
Tecnologías que involucran la manipulación de células, tejidos, órganos o todo el organismo
Biotecnología de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1735