Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage

Authors
Vera, F.; Craig, M. I.; Olivera, V.; Rojas, F.; König, Guido Alberto; Pereda, Ariel Julián; Vagnozzi, Ariel Eduardo
Publication Year
2015
Language
English
Format
article
Status
Published version
Description
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
Fil: Vera, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Area de Producción Animal; Argentina
Fil: Craig, M. I.. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina
Fil: Olivera, V.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Rojas, F.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: König, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereda, Ariel Julián. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Subject
Infectious Bursal Disease Virus
nucleotide sequence identity
Regional lineage
Amino acid sequence analysis
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/38446