Hepatitis C virus genotype 4 in Southern and Central Spain does not come from recent foreign migration waves

Authors
Di Lello, Federico Alejandro; Neukam, Karin; Parra Sanchez, Manuel; Plaza, Zulema; Soriano, Vicente; Cifuentes, Celia; Mira, José Antonio; Poveda, Eva; Pineda, Juan Antonio
Publication Year
2013
Language
English
Format
article
Status
Published version
Description
Hepatitis C virus genotype 4 (HCV-4) is highly prevalent in Spain, but the information on the molecular characterization of HCV-4 in this region is scarce. Due to this, the molecular characteristics and the evolution of HCV-4 infection in Seville were analyzed (Southern Spain) and compared them with samples from Madrid. HCV genotype was determined by LIPA 2.0 assay and confirmed by sequence analysis of NS5B. Phylogenetic tree was estimated by MEGA 5.10. Bayesian coalescent-based methods were used to estimate the substitution rate and the age of the most recent common ancestor (MRCA). In the phylogenetic analysis of 50 NS5B HCV-4 from Seville and 11 from Madrid, 2 clusters were distinguished: The first cluster (HCV-4a) included 48% of the sequences from Seville and 9% of sequences from Madrid. The second cluster included the remaining sequences belonging to HCV-4d. The mean estimated substitution rate was 2.39 × 10−3 for HCV-4a and 1.81 × 10−3 for HCV-4d for Seville and 2.32 × 10−3 for HCV-4d from Madrid. The date for MRCA was estimated to be around 1981–1984 for HCV-4 from Seville. The dates for MRCA were dated before the recent flow of immigration in Spain. Therefore, the results presented in this study argues against the possibility of a foreign introduction of the HCV-4 from other regions with high prevalence, at least during the last two, decades in which there was a great flow of immigrants. Additionally, an unusual high prevalence of subtype 4a was observed in Seville.
Fil: Di Lello, Federico Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Microbiología, Inmunología y Biotecnología. Cátedra de Virología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina; Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Neukam, Karin. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Parra Sanchez, Manuel. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Plaza, Zulema. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Soriano, Vicente. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Cifuentes, Celia. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Mira, José Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Fil: Poveda, Eva. Hospital Carlos III. Departamento de Enfermedades Infecciosas; España;
Fil: Pineda, Juan Antonio. Hospital Universitario de Valme. Unidad de Enfermedades Infecciosas y Microbiología; España;
Subject
HCV
DIVERSITY
PHYLOGENY
BEAST
Enfermedades Infecciosas
Ciencias de la Salud
CIENCIAS MÉDICAS Y DE LA SALUD
Bioquímica y Biología Molecular
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Genética y Herencia
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/1991