HLA-G1 increases the radiosensitivity of human tumoral cells

Authors
Gallegos, Cristina Eugenia; Michelin, Severino; Baffa Trasci, Sofía; Aballay Lobos, Elizabeth; Dubner, Diana; Carosella, Edgardo D.
Publication Year
2014
Language
English
Format
article
Status
Published version
Description
Different molecules regulate the response of tumoral tissues to ionizing radiation. The objective of this work was to determine if HLA-G1 expression modulates the radiosensitivity of human tumoral cell lines. To this end, human melanoma M8 and human erythroleukemia K562 cell lines, with their correspondent HLA-G1 negative and positive variants, were gamma irradiated and the survival frequency was determined by clonogenic assay. The survival fraction of HLA-G1 expressing cells was around 60% of HLA-G1 negative cells. The generation of acidic vesicular organelles was higher in HLA-G1 positive cells. Apoptosis levels showed statistically significant differences only in K562 cells, whereas the variation in G2/M cycle progression was only significant in M8 cells. In addition, irradiation diminished cell-surface HLA-G1 and increased soluble HLA-G1 levels. Soluble HLA-G1 has no influence on cell survival in any cell line. In summary, we could demonstrate that HLA-G1 confers higher radiosensitivity to HLA-G1 expressing cells.
Fil: Gallegos, Cristina Eugenia. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnológico Bahia Blanca. Instituto de Ciencias Biologicas y Biomedicas del Sur; Argentina. Universidad Nacional del Sur; Argentina. Autoridad Regulatoria Nuclear; Argentina
Fil: Michelin, Severino. Autoridad Regulatoria Nuclear; Argentina
Fil: Baffa Trasci, Sofía. Autoridad Regulatoria Nuclear; Argentina
Fil: Aballay Lobos, Elizabeth. Autoridad Regulatoria Nuclear; Argentina
Fil: Dubner, Diana. Autoridad Regulatoria Nuclear; Argentina
Fil: Carosella, Edgardo D.. University Paris Diderot; Francia. Commissariat a l’Energie Atomique et aux Energies Alternatives. Institute of Emerging Diseases and Innovative Therapies. Research Division in Hematology and Immunology; Francia
Subject
HLA-G1
IONIZING RADIATION
RADIOSENSITIVITY
TUMORAL CELLS
MELANOMA CELLS
ERYTHROLEUKEMIA CELLS
Biología Celular, Microbiología
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Inmunología
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/6356