Accelerated cell divisions drive the outgrowth of the regenerating spinal cord in axolotls

Authors
Rost, Fabian; Rodrigo Albors, Aida; Mazurov, Vladimir; Brusch, Lutz; Deutsch, Andreas; Tanaka, Elly M; Chara, Osvaldo
Publication Year
2016
Language
English
Format
article
Status
Published version
Description
Axolotls are unique in their ability to regenerate the spinal cord. However, the mechanisms that underlie this phenomenon remain poorly understood. Previously, we showed that regenerating stem cells in the axolotl spinal cord revert to a molecular state resembling embryonic neuroepithelial cells and functionally acquire rapid proliferative divisions (Rodrigo Albors et al., 2015). Here, we refine the analysis of cell proliferation in space and time and identify a highproliferation zone in the regenerating spinal cord that shifts posteriorly over time. By tracking sparsely-labeled cells, we also quantify cell influx into the regenerate. Taking a mathematical modeling approach, we integrate these quantitative datasets of cell proliferation, neural stem cell activation and cell influx, to predict regenerative tissue outgrowth. Our model shows that while cell influx and neural stem cell activation play a minor role, the acceleration of the cell cycle is the major driver of regenerative spinal cord outgrowth in axolotls.
Fil: Rost, Fabian. Technische Universitat Dresden; Alemania
Fil: Rodrigo Albors, Aida. Center for Regenerative Therapies Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Mazurov, Vladimir. Center for Regenerative Therapies Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Brusch, Lutz. Technische Universitat Dresden; Alemania
Fil: Deutsch, Andreas. Technische Universitat Dresden; Alemania
Fil: Tanaka, Elly M. Center for Regenerative Therapies Dresden; Alemania. Max Planck Institute of Molecular Cell Biology and Genetics; Alemania
Fil: Chara, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Física de Líquidos y Sistemas Biológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Física de Líquidos y Sistemas Biológicos; Argentina. Technische Universitat Dresden; Alemania
Subject
REGENERATION
AXOLOTL
MATHEMATICAL MODELING
SPINAL CORD
Otras Ciencias Biológicas
Ciencias Biológicas
CIENCIAS NATURALES Y EXACTAS
Access level
Open access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/48245