A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds

Authors
Cumashi, Albana; Ushakova, Natalia A.; Preobrazhenskaya, Marina E.; D'Incecco, Armida; Piccoli, Antonio; Totani, Licia; Tinari, Nicola; Morozevich, Galina E.; Berman, Albert E.; Bilan, María; Usov, Anatolii I.; Ustyuzhanina, Nadezhda E.; Grachev, Alexey A.; Sanderson, Craig J.; Kelly, Maeve; Rabinovich, Gabriel Adrián; Iacobelli, Stefano; Nifantiev, Nikolay E.
Publication Year
2007
Language
English
Format
article
Status
Published version
Description
The anti-inflammatory, antiangiogenic, anticoagulant, and antiadhesive properties of fucoidans obtained from nine species of brown algae were studied in order to examine the influence of fucoidan origin and composition on their biological activities. All fucoidans inhibited leucocyte recruitment in an inflammation model in rats, and neither the content of fucose and sulfate nor other structural features of their polysaccharide backbones significantly affected the efficacy of fucoidans in this model. In vitro evaluation of P-selectin-mediated neutrophil adhesion to platelets under flow conditions revealed that only polysaccharides from Laminaria saccharina, L. digitata, Fucus evanescens, F. serratus, F. distichus, F. spiralis, and Ascophyllum nodosum could serve as P-selectin inhibitors. All fucoidans, except that from Cladosiphon okamuranus carrying substantial levels of 2-O-alpha-D-glucuronopyranosyl branches in the linear (1-->3)-linked poly-alpha-fucopyranoside chain, exhibited anticoagulant activity as measured by activated partial thromboplastin time whereas only fucoidans from L. saccharina, L. digitata, F. serratus, F. distichus, and F. evanescens displayed strong antithrombin activity in a platelet aggregation test. The last fucoidans potently inhibited human umbilical vein endothelial cell (HUVEC) tubulogenesis in vitro and this property correlated with decreased levels of plasminogen-activator inhibitor-1 in HUVEC supernatants, suggesting a possible mechanism of fucoidan-induced inhibition of tubulogenesis. Finally, fucoidans from L. saccharina, L. digitata, F. serratus, F. distichus, and F. vesiculosus strongly blocked MDA-MB-231 breast carcinoma cell adhesion to platelets, an effect which might have critical implications in tumor metastasis. The data presented herein provide a new rationale for the development of potential drugs for thrombosis, inflammation, and tumor progression.
Fil: Cumashi, Albana. Universita degli Studi G. D Annunzio; Italia
Fil: Ushakova, Natalia A.. Academia Rusa de Ciencias Médicas; Rusia
Fil: Preobrazhenskaya, Marina E.. Academia Rusa de Ciencias Médicas; Rusia
Fil: D'Incecco, Armida. Universita degli Studi G. D Annunzio; Italia
Fil: Piccoli, Antonio. Consorzio Mario Negri Sud; Italia
Fil: Totani, Licia. Consorzio Mario Negri Sud; Italia
Fil: Tinari, Nicola. Universita degli Studi G. D Annunzio; Italia
Fil: Morozevich, Galina E.. Academia Rusa de Ciencias Médicas; Rusia
Fil: Berman, Albert E.. Academia Rusa de Ciencias Médicas; Rusia
Fil: Bilan, María. Academia Rusa de Ciencias; Rusia
Fil: Usov, Anatolii I.. Academia Rusa de Ciencias; Rusia
Fil: Ustyuzhanina, Nadezhda E.. Academia Rusa de Ciencias; Rusia
Fil: Grachev, Alexey A.. Academia Rusa de Ciencias; Rusia
Fil: Sanderson, Craig J.. Scottish Association for Marine Sciences; Reino Unido
Fil: Kelly, Maeve. Scottish Association for Marine Sciences; Reino Unido
Fil: Rabinovich, Gabriel Adrián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad de Buenos Aires. Facultad de Medicina. Hospital de Clínicas General San Martín; Argentina
Fil: Iacobelli, Stefano. Universita degli Studi G. D Annunzio; Italia
Fil: Nifantiev, Nikolay E.. Academia Rusa de Ciencias; Rusia
Subject
FUCOIDAN
BROWN ALGAE
BLOOD COAGULATION
INFLAMMATION
ANGIOGENESIS
Bioquímica y Biología Molecular
Medicina Básica
CIENCIAS MÉDICAS Y DE LA SALUD
Access level
Restricted access
License
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repository
CONICET Digital (CONICET)
Institution
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identifier
oai:ri.conicet.gov.ar:11336/29419